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siRNA transfection protocols, technical considerations, resources and references are summarized. siRNA are 21-25 bp size double-stranded short RNA molecules that performs fundamental functions within cells and is becoming an important reverse genetics tool to study gene functions.
Plasmid DNA purification techniques for extraction and purification of plasmid DNA from host bacteria.
DNA techniques on transformation, cDNA and genomic libraries construction, probe labeling and purification, gene expression profiling analysis, in vitro mutagenesis, and DNA sequencing.
DNA quantification methods overview. Some commonly used DNA quantification methods and related resources. DNA quantification methods include UV spectrometry, fluorescent DNA-binding dyes, agarose gel electrophoresis and staining, real-time PCR etc.
