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TRAzol

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Product/service: TRAzol 
Unit Price:   Inquiry 
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Delievery time: Within 3 days upon receipt of payment
Valid through: Not expired
Last update on: 2012-10-29
View times: 926
Company details
  • Dongsheng Biotech Co., Ltd
  • Contacthellen fung   
  •    Not registered
  • Tel  
  • Location  China
  • Address  #610, Yanqiao Mansion, 89 Yanling Road, Tianhe District, Guangzhou, Guangdong, China
 
 
Product details

TRAzol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method. During sample homogenization or lysis, TRAzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase.

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