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rat adrencocorticotropic hormone,ACTH ELISA Kit

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Product/service: rat adrencocorticotropic hormone,ACTH ELISA Kit 
Brand: USCNLIFE 
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Delievery time: Within 3 days upon receipt of payment
Valid through: Not expired
Last update on: 2011-01-09
View times: 1650
Company details
  • Wuhan EIAab Science Co., Ltd.
  • Contactchen lu       
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  • Location  China
  • Address  A1710 Guangguguoji,East Lake Hi-Tech Zone,Wuhan 430079,P. R. China
 
 
Product details

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat adrencocorticotropic hormone,ACTH concentrations in serum, plasma and other biological fluids.
 
Introduction
ACTH (Adrenocorticotropic hormone) or corticotropin is a 39-amino acid peptide hormone (MW=4500) secreted by the pituitary to regulate the production of steroid hormones by the adrenal cortex. ACTH secretion from the anterior pituitary is controlled by both a classical negative feedback control mechanism and CNS-stress mediated control system. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH), a 41-amino acid peptide. CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation. ACTH increases the synthesis and release of all adrenal sterioids, aldosterone, cortisol and adrenal androgens. It is the principal modulator of cortisol, the most important glucocorticoid in man. As the cortisol level in blood increases, release of ACTH is inhibited directly at the pituitary level. Through this same mechanism, decreasing cortisol levels lead to elevated ACTH levels. Biologically active ACTH results from enzymatic cleavage of a large precursor molecule, proopiomelanocortin (POMC). This molecule contains within its structure the amino acid sequences of ACTH, Pro-ACTH, -melanocyte stimulating hormone, lipotropin, as well as endorphin and the enkephalins.
 
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACTH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ACTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ACTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of ACTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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