This immunoassay kit allows for the in vitro quantitative determination of rat MT concentrations in serum, plasma and other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of MT in the samples is then determined by comparing the O.D. of the samples to the standard curve.