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ELISA Kit

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Product/service: Canine Interleukin 1β,IL-1β ELISA Kit 
Unit Price: 4500 USD 96t  Inquiry 
Minimum quantity:   Place order 
Supply ability:
Delievery time: Within 3 days upon receipt of payment
Valid through: Not expired
Last update on: 2010-11-18
View times: 1367
Company details
  • WuHan EIAab Science Co Ltd
  • ContactYuki.Cai     
  •    Not registered
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  • Location  China
  • Address  A1710 Guangguguoji,East Lake Hi-Tech Development Zone,Wuhan 430079,China
 
 
Product details

Intended use
This immunoassay kit allows for the in vitro quantitative determination of canine Interleukin 1β,IL-1β concentrations in cell culture supernates, serum, plasma and other biological fluids.
 
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1β. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-1β. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-1β, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IL-1β in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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