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ELISA Kit

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Product/service: Bovine α Lactalbumin,α-La ELISA Kit  
Brand: Uscnlife 
Size: 96t 
Unit Price: 4500 USD 96t   
Minimum quantity:    
Supply ability:
Delievery time: Within 1 days upon receipt of payment
Valid through: 2011-01-31 [Expired]
Last update on: 2010-11-18
View times: 535
Company details
  • WuHan EIAab Science Co Ltd
  • ContactYuki.Cai     
  •    Not registered
  • email  
  • Tel  
  • Location  China
  • Address  A1710 Guangguguoji,East Lake Hi-Tech Development Zone,Wuhan 430079,China
 
 
Product details

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine α-La concentrations in serum, plasma and other biological fluids.
 
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to α-La. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for α-La. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain α-La, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of α-La in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 
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