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cAMP Direct Immunoassay Kit, Colorimetric

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Product/service: cAMP Direct Immunoassay Kit, Colorimetric 
Model: 116811-1KIT 
Size: 1 kit 
Unit Price: 479 USD   Inquiry 
Minimum quantity´╝Ü    
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Delievery time: Within days upon receipt of payment
Valid through: Not expired
Last update on: 2010-05-29
View times: 847
Company details
  • EMD Chemicals
  • ContactEMD Chemicals Inc.   
  •    Not registered
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  • Location  USA
  • Address  Gibbstown, NJ 08027, USA
Product details

Assay range: 0.78 - 200 pmol/ml (non-acetylated), 0.078-20 pmol/ml (acetylated)

Assay time 4.5 h

Detection method Colorimetric

Form 100 Tests

Format 96-well format

Kit contents:
A non-radioactive competitive immunoassay kit for the rapid quantitative measurement of cAMP. No neutralization, extraction, drying, or reconstitution of samples is required. Samples are treated with 0.1N HCl to lyse the cells, stop endogenous phosphodiesterase activity, and stabilize the released cAMP, which can then be analyzed directly in the microtiter plate.

Chard, T. In: An Introduction to Radioimmunoassay and Related Techniques. 1990. 4th Ed., Elsevier, Amsterdam.NCCLS Evaluation Protocols, SC1, 1989, NCCLS, Villanova, PA, 19085.Tijssen, P. In: Practice and Theory of Enzyme Immunoassays. 1985. Elsevier, Amsterdam.Chabardes, D., et al. 1980. J. Clin. Invest. 65, 439.Grill, V., and Cerasi, E. 1974. J. Biol. Chem. 249, 41961.Szentivanyi, A. 1968. J. Allergy 42, 203.Haynes, R.C. 1958. J. Biol. Chem. 233, 1220.

Sample type Serum, cell, and tissue samples

Sensitivity 390 fmol/ml (non-acetylated), 37 fmol/ml (acetylated)

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