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ProteoExtract® Partial Mammalian Proteome Extraction Kit

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Product/service: ProteoExtract® Partial Mammalian Proteome Extraction Kit 
Model: 539789-1KIT 
Size: 1 kit 
Unit Price: 349 USD   Inquiry 
Minimum quantity:    
Supply ability:
Delievery time: Within days upon receipt of payment
Valid through: Not expired
Last update on: 2011-01-15
View times: 1207
Company details
  • EMD Chemicals
  • ContactEMD Chemicals Inc.   
  •    Not registered
  • email  
  • Tel  
  • Location  USA
  • Address  Gibbstown, NJ 08027, USA
 
 
Product details

Assay range:

Assay time

Detection method

Form 20 Extractions

Format

Kit contents:
The Partial Mammalian Proteome Extraction Kit (P-PEK, Mammalian) is designed for serial sample preparation of complex mixtures using reagents with increasing solubilization strength. Each of the provided extraction reagents solubilizes a different subset of cell proteins. The most soluble of the mammalian proteins are released by mechanical destruction of the cells in Extraction Reagent 1, proteins of intermediate solubility are subsequently extracted with reagent 2, then the membrane proteins can be extracted using Extraction Reagent 3. These fractions can then be analyzed on separate 2-D gel electrophoresis and the insoluble fraction can be analyzed by one-dimensional SDS-PAGE using the SDS buffers provided. To preserve the protein profile and increase spot resolution, the kit also contains Protease Inhibitor Cocktail and the proprietary non-specific nuclease, Benzonase® Nuclease (Cat. No. 71206). Sample size: 1-2x108 cells or 500 mg tissue.

References
Yuan, X., et al. 2002. Electrophoresis 23, 1185.Link, A.J. 1999. 2D Proteome Analysis Protocols Humana Press.Wilkins, M.R., et al. 1997. Proteome Research: New Frontiers in Functional Genomics Springer-Verlag.

Sample type Mammalian cells

Sensitivity
http://www.emdchemicals.com/products/EMD_BIO-539789?WT.mc_id=EB000129

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