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Protein Kinase Assay Kit, Non-Radioactive

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Product/service: Protein Kinase Assay Kit, Non-Radioactive 
Model: 539484-1KIT 
Size: 1 kit 
Unit Price: 549 USD   Inquiry 
Minimum quantity´╝Ü    
Supply ability:
Delievery time: Within days upon receipt of payment
Valid through: Not expired
Last update on: 2010-05-29
View times: 774
Company details
  • EMD Chemicals
  • ContactEMD Chemicals Inc.   
  •    Not registered
  • email  
  • Tel  
  • Location  USA
  • Address  Gibbstown, NJ 08027, USA
 
 
Product details

Assay range:

Assay time

Detection method Colorimetric

Form 96 Tests

Format 96-well plate

Kit contents:
Useful for measuring protein kinase A (PKA) or protein kinase C (PKC) activity in crude cell extracts or semi-purified enzyme preparations. The assay kit is based on an ELISA method which utilizes a synthetic PKA/PKC pseudosubstrate and a monoclonal antibody which recognizes the phosphorylated form of that peptide. May be used for screening inhibitors and activators of PKA and/or PKC. Supplied with a user protocol.

References
Newton, A.C. 1995. J. Biol. Chem. 270, 28495.Nishizuka, Y. 1995. FASEB J. 9, 484.Nishizuka, Y. 1988. Nature 334, 661.Edelman, A.M., et al. 1987. Ann. Rev. Biochem. 56, 567.Nishizuka, Y. 1986. Science 233, 305.Montimity, M.R. and Bilezikijan, L.M. 1987. Nature 56, 175.Inagaki, M., et al. 1985. J. Biol. Chem. 260, 2922.Wolf, M., et al. 1985. Nature 317, 546.Beavo, J.A., et al 1974. Methods Enzymol. 38, 299.

Sample type Cell lysates, purified enzymes

Sensitivity
http://eshop.emdchemicals.com/products/EMD_BIO-539484?WT.mc_id=EB000129

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