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Proteasome Isolation Kit, Human

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Product/service: Proteasome Isolation Kit, Human 
Model: 539176-1KIT 
Size: 1 kit 
Unit Price: 499 USD   Inquiry 
Minimum quantity´╝Ü    
Supply ability:
Delievery time: Within days upon receipt of payment
Valid through: Not expired
Last update on: 2010-05-29
View times: 628
Company details
  • EMD Chemicals
  • ContactEMD Chemicals Inc.   
  •    Not registered
  • email  
  • Tel  
  • Location  USA
  • Address  Gibbstown, NJ 08027, USA
 
 
Product details

Assay range:

Assay time 3-5 h

Detection method

Form

Format 1.0 ml bead suspension

Kit contents:
A rapid method for the isolation of biologically active proteasomes using affinity matrix beads comprised of a GST-fusion protein containing an ubiquitin-like domain (UbL) bound to GST-agarose. The proteasome subunit proteins can be identified by loading the beads directly onto an SDS-PAGE gel and immunoblotting with subunit specific antibodies. Alternatively, proteasome bound to beads can be used in proteolytic assays using proteasome substrates.

Utilize Ubiquitin-like domain (Ubl) bound to GST-agarose
Rapidly isolate biologically active proteasomes
Affinity mature beads can be directly loaded onto SDS-PAGE gels for proteasome subunit identification
Proteasome-bound beads are compatible with proteolytic assays using proteasome substrates

References
Chen, L., and Madura, K. 2002. Mol. Cell Biol. 22, 4902.Chen, L., et al. 2001. EMBO Rep. 2, 933.Schauber, C., et al. 1998. Nature 391, 715.

Sample type Cell extracts

Sensitivity
http://eshop.emdchemicals.com/products/EMD_BIO-539176?WT.mc_id=EB000129

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