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Glycoprotein Deglycosylation Kit

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Product/service: Glycoprotein Deglycosylation Kit 
Model: 362280-1KIT 
Size: 1 kit 
Unit Price: 549 USD   Inquiry 
Minimum quantity´╝Ü    
Supply ability:
Delievery time: Within days upon receipt of payment
Valid through: Not expired
Last update on: 2010-05-29
View times: 676
Company details
  • EMD Chemicals
  • ContactEMD Chemicals Inc.   
  •    Not registered
  • email  
  • Tel  
  • Location  USA
  • Address  Gibbstown, NJ 08027, USA
 
 
Product details

Assay range:

Assay time 3 h-5 days

Detection method

Form

Format

Kit contents:
Kit contains all enzymes and reagents needed to remove all N-linked, all simple O-linked, and virtually all-complex O-linked oligosaccharides from glycoproteins in a single reaction at neutral pH. Suitable for use with native or denaturation protocols, with no degradation of protein. This kit has been assembled to contain sequencing grade enzymes without azide, BSA, or glycerol. Each kit can be used to deglycosylate up to 2 mg glycoprotein.

References
Chiba, A., et al. 1997. J. Biol. Chem. 272, 2156.Jiang, M.S., and Hart, G.W. 1997. J. Biol. Chem. 272, 2421.Wilkins, P.P., et al. 1996. J. Biol. Chem. 271, 18732.Altmann, F., et al. 1995. Glycoconj. J. 12, 84.Szkudlinski, M.W., et al. 1995.Endocrinology 136, 3325.Pahlsson, P., et al. 1994. Glycoconj. J. 11, 43.Saito, S., et al. 1994. J. Biol. Chem. 269, 5644.Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.Iwase, H., Hotta, K. 1993. Methods. Mol. Biol. 14, 151.Stults, N.L., and Cummings, R.D. 1993. Glycobiology 3, 589.Hard, K., et al. 1992. Eur. J. Biochem. 205, 785.Kuraya, N., and Hase, S. 1992. J. Biochem. (Tokyo) 112, 122.Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.Fukuda, M., et al. 1987. J. Biol. Chem. 262, 11952.Sojar, H.T., and Bahl, O.P. 1987. Arch. Biochem. Biophys. 259, 52.Taga, E.M., et al. 1984. Biochemistry 23, 815.Kobata, A. 1979. Anal. Biochem. 100, 1.Uchida, Y., et al. 1979. J. Biochem (Tokyo) 86, 1573.Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.Spiro, R.G., and Bhoyroo, V.D. 1974. J. Biol. Chem. 249, 5704.

Sample type

Sensitivity
http://eshop.emdchemicals.com/products/EMD_BIO-362280?WT.mc_id=EB000129

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