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Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux® G2D2)

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Product/service: Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux® G2D2) 
Model: 235432-1KIT 
Size: 1 kit 
Unit Price: 499 USD   Inquiry 
Minimum quantity´╝Ü    
Supply ability:
Delievery time: Within days upon receipt of payment
Valid through: Not expired
Last update on: 2010-05-29
View times: 846
Company details
  • EMD Chemicals
  • ContactEMD Chemicals Inc.   
  •    Not registered
  • email  
  • Tel  
  • Location  USA
  • Address  Gibbstown, NJ 08027, USA
 
 
Product details

Assay range:

Assay time 1.5-2 h

Detection method Fluorescence

Form 30 Tests

Format Flow cytometry or fluorescence microscopy

Kit contents:
Assay kit for the determination by flow cytometry or fluorescence microscopy of caspase-3-like activity in intact cells undergoing apoptosis. Can be used to evaluate the ability of a test substance or drug candidate to activate or inhibit caspase-3. The assay is based on the reaction of the enzyme with the cell-permeable PhiPhiLux® G2D2 substrate. Each substrate molecule contains two fluorophores and the cleaved substrate fluoresces red.

References
Chang, S.H., et al. 2002. Exp. Cell Res. 277, 15.Kottke, T.J., et al. 2002. J. Biol. Chem. 277, 804.Liu, L., et al. 2002. Nat. Med. 8, 185.Telford, W.G., et al. 2002. Cytometry 47, 81.Packard, B.Z., et al. 2001. J. Immunol. 167, 5061.Finucane, D.M., et al. 1999. J. Biol. Chem. 274, 2225.Porter, A.G. and Janicke, R.U. 1999. Cell Death Differ. 6, 99.Robles, R., et al. 1999. Endocrinology 140, 2641.Hirata, H., et al. 1998. J. Exp. Med. 187, 587.Packard, B.Z., et al. 1998. J. Phys. Chem. B 102, 1820.Siegel, R.M., et al. 1998. J. Cell Biol. 141, 1243.Packard, B.Z., et al. 1997. Methods Enzymol. 278, 15.Nicholson, D.W., et al. 1995. Nature 376, 37.

Sample type Intact cells

Sensitivity
http://eshop.emdchemicals.com/products/EMD_BIO-235432?WT.mc_id=EB000129

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