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Akt Activity Immunoassay Kit

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Product/service: Akt Activity Immunoassay Kit 
Model: 124007-1KIT 
Size: 1 kit 
Unit Price: 499 USD   Inquiry 
Minimum quantity´╝Ü    
Supply ability:
Delievery time: Within days upon receipt of payment
Valid through: Not expired
Last update on: 2011-01-15
View times: 1205
Company details
  • EMD Chemicals
  • ContactEMD Chemicals Inc.   
  •    Not registered
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  • Location  USA
  • Address  Gibbstown, NJ 08027, USA
Product details

Assay range:

Assay time 4 h

Detection method Immunoblot

Form 40 Tests

Format Immunoblot

Kit contents:
Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.

Nickolson, K.M., and Anderson, N.G. 2002. Cell Signal. 14, 381.van Weeren, P.C., et al. 1998. J. Biol. Chem. 273, 13150.

Sample type Cell lysates, tissue extracts


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