Categories (1-5 of 6)
| PCR: Degenerate PCR |
| Cloning and subcloning |
| DNA: DNA mutagenesis |
| Cloning and subcloning: Cloning vectors |
| Cloning and subcloning: PCR cloning |
Life science protocols and methods » Search Results
Featured Protocols
| PCR primer design software and online tools - http://methods-techniques.org/pcr/ Category: PCR: PCR primer design Online PCR primer design tools
|
Protocols Sort by: Hits | Alphabetical
| Sandwich ELISA - http://www.komabiotech.com/technical/protocol/ELISAsand.htm Category: ELISA: Sandwich ELISA Sandwich ELISA. The following is a general protocol for a sandwich (capture) ELISA . The precise conditions should be optimized for a particular assay. ... |
| Competitive ELISA assays, Competitive ELISA protocol ... - http://www.millipore.com/cellbiology/cb3/competitiveelisamethod Category: ELISA: Competitive ELISA Competitive ELISA assays, Competitive ELISA protocol and Competitive ELISA method - General Protocol for the Competitive ELISA Method. |
| Cell Migration Assay - http://www.mae.ufl.edu/cellm Category: Cell culture: Cell Migration Assay Designing A New Cell Migration Assay. Cellular migration is fundamental to various in vivo processes including organogenesis, metastasis, and wound healing . ... |
| DNA Ligation Protocol - http://www.hawaii.edu/microbiology/MO/ligation.htm Category: Cloning and subcloning: DNA ligation Jul 21, 2003 ... A. One Hour Protocol For Subcloning and Size-Fractionated Deletion Cloning B. Three-Minute Ligation. I. Description. The DNA Ligation Kit is ... |
| DNA dephosphorylation - http://dna.dephosphorylation Category: Cloning and subcloning: DNA dephosphorylation Vector dephosphorylation step Dephosphorylation step is necessary to prevent self-ligation of cloning vectors in blunt-end and single restriction enzyme cloning. Calf intestinal alkaline phosphatase (CIAP) is most frequently used to remove 5' phosphate groups from cloning vector. To calculate the amount pmol of ends for linear dsDNA, use equation bellow. (ug DNA/(kb size of DNA))*3.04 |
| Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes - OpenWetWare - http://openwetware.org/wiki/Koch_Lab:Protocols/Oligonucleotide_Annealing/Duplexes Category: Oligonucleotide: Oligonucleotides annealing Jul 15, 2008 ... Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes ... General protocol. Mix oligos together at high concentration in a buffer with ... |
| Elisa Protocol - ELISA Protocol - http://www.elisaprotocol.com/ Category: ELISA: Sandwich ELISA ELISA Articles � General ELISA � Sandwich ELISA ... Elisa Protocol - ELISA Protocol |
| Methylation Specific PCR - http://www.epigeneticstation.com/methylation-specific-pcr/ Category: PCR: Methylation specific PCR METHODS and MATERIALS: A Protocol for Methylation-Specific PCR, MSP. One micro g of sodium bisulfite-treated genomic DNA is used for PCR amplification using ... |
| Nature Protocols: Tissue subcellular fractionation and protein extraction - http://www.natureprotocols.com/2006/11/22/tissue_subcellular_fractionati.php Category: Proteomics: Protein extraction and purification: Protein extraction Tissue subcellular fractionation and protein extraction for use in ... The protocol described is for the general fractionation and extraction of proteins ... |
| Mutagenesis methods protocols and methods - http://biowww.net/methods/browse-10.html Category: DNA: DNA mutagenesis A simple and efficient method for chemical mutagenesis of DNA. full-text paper ... An efficient protocol for linker scanning mutagenesis: analysis of the ... |
| PCR cloning - a protocol - http://www.bio.net/bionet/mm/methods/1994-March/012356.html Category: Cloning and subcloning: PCR cloning PCR cloning - a protocol. txpljfg at UABCVSR.CVSR.UAB.EDU txpljfg at UABCVSR. CVSR.UAB.EDU Fri Mar 11 15:31:42 EST 1994. Previous message: plastic disposable ... |
| Suspension cell culture - http://biowww.net/detail-1373.html Category: Cell culture: Suspension cell culture Oct 16, 2008 ... Suspension cell culture protocol and general procedure. |
| Ligation and Transformation Protocol - http://biowww.net/detail-1045.html Category: Cloning and subcloning: Transformation screening May 12, 2005 ... Protocol on subcloning ligation and transformation. (Tim Fitzwater, SomaLogic, Inc, posted on U.S. Dept commerce/NOAA/NMFS/NWFSC/Molecular ... |
| Nuclear Run-On Assay - http://www.iscid.org/encyclopedia/Nuclear_Run-On_Assay Category: RNA: Nuclear Run-On Assay Encyclopedia information on Nuclear Run-On Assay. ... Nuclear run-on assays are transcription assays designed to look at the genes being transcribed in a ... |
| Dephosphorylation of DNA Fragments with Alkaline Phosphatase - http://cshprotocols.cshlp.org/cgi/content/full/2006/2/pdb.prot3863?print=true Category: Cloning and subcloning: DNA dephosphorylation Dephosphorylation of DNA Fragments with Alkaline Phosphatase. Joseph Sambrook and David W. Russell. This protocol was adapted from Molecular Cloning, ... |
| [PDF] PCR cloning protocol - http://www.ebiotrade.com/buyf/productsf/qiagen/1016726HBDNYPCRCloning401WW.pdf Category: Cloning and subcloning: PCR cloning PCR cloning protocol File Format: PDF/Adobe Acrobat |
| Oxford Laboratory Technology: Agarose Gel Electrophoresis-Protocol - http://labclone.blogspot.com/2007/06/agarose-gel-electrophoresis-protocol.html Category: Electrophoresis: DNA electrophoresis Always run the DNA toward the red (+) terminal. Electrophoresis the gel at 11 volts overnight or ... Protocol for CopyControl’ BAC Cloning Kit - 25-Oct-2007 ... |
| Life Or Death Decisions: How To Choose A Cytotoxicity Assay - http://www.biocompare.com/Articles/BuyingTip/14/Life-Or-Death-Decisions-How-To-Choose-A-Cytotoxicity-Assay.html Category: Cell culture: Cytotoxicity assay Life Or Death Decisions: How To Choose A Cytotoxicity Assay Cell cultures are often used as stand-ins for more integral systems. They are generally easier ... |
| Free cloning vector maps Download - cloning vector maps Software - http://wareseeker.com/free-cloning-vector-maps/ Category: Cloning and subcloning: Cloning vectors Free download cloning vector maps Software at WareSeeker.com - Raster to Vector is a stand-alone program that converts scanned drawings, maps and raster ... |
| General cell culture protocol - http://biowww.net/detail-1301.html Category: Cell culture Oct 10, 2007 ... Cell culture, tissue culture general protocol and introduction. |
| PCR-Degenerate Protocol - http://methods2protocols.com/category/pcr-degenerate-protocol-methods/ Category: PCR: Degenerate PCR Posted by Team Methods2Protocols | Filed Under Molecular Biology Protocol- Methods, PCR-Degenerate Protocol-Methods | Leave a Comment ... |
| PCR Cloning Protocols - http://www.biocompare.com/protocols/category/3120/PCR-Cloning.html Category: Cloning and subcloning: PCR cloning PCR Cloning Protocols Protocol Details. ... Bidirectional Cloning Of PCR Products (Subscription Required). Source: CSH Protocols; 2006; doi:10.1101/pdb. ... |
| DNA Ligation - http://www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/ligation.html Category: Cloning and subcloning: DNA ligation Joining linear DNA fragments together with covalent bonds is called ligation. More specifically, DNA ligation involves creating a ... |
| Site-directed mutagenesis - OpenWetWare - http://openwetware.org/wiki/Site-directed_mutagenesis Category: DNA: DNA mutagenesis Purify template plasmid DNA from a dam+ Escherichia coli strain (to ensure ... See my 'Round-the-horn site-directed mutagenesis protocol to get around these ... |
| RNA electrophoresis - http://www.genelink.com/siRNA/rnaiwhatis.asp?id=26 Category: Electrophoresis: RNA electrophoresis Please consult the transfection reagent vendor’s protocol for detailed .... rna electrophoresis for demanding applications and consistent results. ... |
| [DOC] RNA extraction and purification protocol - http://greengenes.lbl.gov/Download/Protocols/Total%20Soil%20DNA%20and%20RNA%20extraction%20and%20purificationREVISED.doc Category: RNA: RNA extraction and purification RNA extraction and purification protocol (DOC) |
| DNA Ligation Protocol - http://biologicalworld.com/ligations.htm Category: Cloning and subcloning: DNA ligation DNA Ligation Protocol. Biochemistry, Molecular Biology, and Cell Biology Protocols >> Performing DNA Ligations for Molecular Cloning - Ligation Protocol ... |
| [PDF] Qiagen PCR purification manual - http://molecool.wustl.edu/krolllab/Kroll_Lab_Protocols/Molecular%20Biology%20protocols/Cloning%20protocols%20folder/PCR%20purification-Qiagen.pdf Category: PCR: PCR purification Qiagen PCR purification manual File Format: PDF/Adobe Acrobat |
| Protocol for Western Blotting of Tissue Culture Cells - http://www.thelabrat.com/protocols/westernblot.shtml Category: Proteomics: Western blotting Generic protocol for Western Blotting of cells grown in culture. |
| Cloning PCR-Generated DNA Fragments - http://wheat.pw.usda.gov/~lazo/methods/lazo/pcr-cloning.html Category: Cloning and subcloning: PCR cloning Protocol 2, PCR cloning, increases the yield and efficiency of blunt-ended cloning by incorporating a unit excess of restriction enzyme (compared to the ... |
| Embryonic stem cell culture - basic procedures - http://www.molgen.mpg.de/~soldatov/protocols/protocol/02/02_02.htm Category: Cell culture: ES stem cell culture Embryonic stem cell culture - basic procedures. This protocol does not aim at substituting the reading of other general ES cell culture protocols in the ... |
| Transfection Protocols and Applications Guide - http://www.promega.com/paguide/chap12.htm Category: Cell culture: Transient transfection Calcium phosphate transfection is routinely used for both transient and .... of Transfection Efficiency and General Transfection Protocol for details. ... |
| Cloning vector database - http://biowww.net/detail-621.html Category: Cloning and subcloning: Cloning vectors Sep 24, 2008 ... Cloning vector database currently contains 386 entries for various vectors with maps and accession ID. (S. Yasuda, Department of Microbial ... |
| Southern Blot - http://a32.lehman.cuny.edu/molbio_course/blot_protocol.htm Category: DNA: DNA southern blotting hybridization 3. Agarose gel electrophoresis and Southern blotting of DNA. 4. Southern hybridization to identify the gene of interest. Read: Roche Dig-detection protocol ... |
| Map of cloning vector pBS(-) - http://www.bio.net/hypermail/methds-reagnts/1995-October/035080.html Category: Cloning and subcloning: Cloning vectors Is there anybody outside who can give me the sequence map of the cloning vector pBS(-)? I think it s from Stratagene. In their catalog is the pBluescriptII ... |
| [PDF] Site directed mutagenesis protocol - http://www.its.caltech.edu/~bjorker/Protocols/Site-directed_mutagns_pro.pdf Category: DNA: DNA mutagenesis Site directed mutagenesis protocol File Format: PDF/Adobe Acrobat |
| DNAWorks: an automated method for designing oligonucleotides for annealing - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=115297 Category: Oligonucleotide: Oligonucleotides annealing Those oligonucleotides are characterized by highly homogeneous melting .... a parameter called ‘temperature’ in the simulated annealing protocol decreases). ... |
| Nuclear run-on assay - http://en.wikipedia.org/wiki/Nuclear_run-on Category: RNA: Nuclear Run-On Assay A nuclear run-on assay is conducted to identify the genes that are being transcribed at a certain time. Cell nuclei are isolated rapidly, ... |
