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Probe synthesis for in situ hyb

Zoom+  Zoom- Publish date: 2014-06-27   Hits: 664
This is essentially according to the method recommended by Boehringer Mannheim. Cut 20 ug of plasmid DNA with appropriate enzyme in 100 ul. Pheno1, phenol/chloroform, chloroform and ethanol precipitate. Dissolve in 15 ul ddH20.(Hiroyuki Takeda lab, National institute of Genetics).
 
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