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Detection of mutations by RNAse cleavage

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Protocol from Great Ormond Street Children's Hospital. This method is based on the fact that many ribonucleases will cleave single stranded RNA and therefore will cleave DNA:RNA or RNA:RNA hybrids in heteroduplexes where there is a mismatched base. Cleavage of RNA is thought to occur 3¡¯ to pyrimidines and of the 12 possible types of mismatches, four are recognized more efficiently (C:A, C:C, C:T, U:T). The sequence surrounding the mismatch may be important in determining the efficiency of cleavage.
 
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