Listed below are recommended protocols and reagents for the in vitro culture of murine ES cells using ESGRO®. Included are protocols for the exclusive use of ESGRO® and for the use of ESGRO® in combination with a primary mouse embryonic fibroblast feeder layer. The choice of method is dependent upon the ES cell line used. Certain ES cell lines require the use of feeder cells whilst other such as E14TG2a and R1 can be successfully used without feeder cells. Exclusive use of ESGRO® eliminates the need to separate ES cells from feeder cells prior to blastocyst injection.
Nature Protocols 3, 1013 - 1017 (2008) http://www.nature.com/nprot/journal/v3/n6/full/nprot.2008.65.html
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ES cell culture protocol
Publish date: 2014-06-27 Hits: 197
Listed below are recommended protocols and reagents for the in vitro culture of murine ES cells using ESGRO®. Includ