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Flow cytometry protocols

Zoom+  Zoom- Publish date: 2014-06-27   Hits: 1226
Flow cytometry protocols from University of Pennsylvania, school of dental medicine. content: Direct Surface Immunophe
Flow cytometry protocols from University of Pennsylvania, school of dental medicine. content: Direct Surface Immunophenotyping PI Staining for Cell Cycle Determination PLUS FITC-Surface Staining Three Apoptosis Detection Techniques Hoechst/7-AAD TUNEL Annexin 1% Paraformaldehyde Solution for Surface Immunophenotyping 4% Paraformaldehyde Solution for Cytoplasmic Staining, Including TUNEL Permeablizing Solution for Intracellular Staining Some Intracellular Staining Protocols Intracellular bcl-2 Staining of Lymphocytes.

http://biochem.dental.upenn.edu/~sdmfacs/#proto



Other resources:

Flow cytometry protocol from U.Iowa: http://www.healthcare.uiowa.edu/corefacilities/FlowCytometry/protocols/index.htm
  • Preparing Cell Samples for Flow Cytometry
  • Detaching Adherent Cells from Tissue Plates without Trypsin
  • Staining for Viability
  • Cell Fixation
  • Disaggregation of Clumped Cells
  • Immunofluorescence Staining
  • Calcium Flux
  • Apoptosis
  • DNA Content and Cell Cycle
  • Intracellular Staining
  •  
 
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