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Neural Stem Cell Culture and Cryopreservation

Zoom+  Zoom- Publish date: 2014-06-27   Hits: 700
Neural stem cell culture protocol and cryprotection method.
Open access protocol for neural stem cell culture, its microscopy analysis and cryopreservation from Nature Protocols (Laura Pacey KK et al, McMaster University). The protocol gives detail to isolate and expand neural stem cells in the form of neurospheres from tissue dissections of the post-natal mouse brain. Procedures for the long term passage of neurospheres and the cryopreservation of neurospheres are also provided.
 
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