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Fluorescence Microscopy Optimization and Troubleshooting

Zoom+  Zoom- Publish date: 2011-03-18   Source: Olympus Microscopy Resource Ce   Author: Olympus   Hits: 730
Fluorescence microscopy optimization tips including image brightness, image resolution. It also includes a troubleshooti
Fluorescence microscopy optimization tips including image brightness, image resolution. It also includes a troubleshooting guidance table. (Olympus)

A key feature of fluorescence microscopy is its ability to detect fluorescent objects that are sometimes faintly visible or even very bright relative to the dark (often black) background. In order to optimize this feature, image brightness and resolution must be maximized using the principles that will be discussed in this portion of the primer.

http://www.olympusmicro.com/primer/techniques/fluorescence/images/numaper.jpg

Reference:
http://www.olympusmicro.com/primer/techniques/fluorescence/troubleshoot.html
 
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