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Compensation in Flow Cytometry Analyses

Zoom+  Zoom- Publish date: 2014-06-27   Hits: 809
This method review is written by Mario Roederer. Compensation is probably the least understood process accompanying flow cytometric analyses. Perhaps this is because it is often described with the linear algebra elements needed for its computation, and many of us have forgotten what linear algebra is, much less how to do it. However, proper compensation is absolutely crucial for some aspects of flow cytometry, one of which is antigen density measurements. Unfortunately, because compensation is often misunderstood, and because there is so much incorrect mythology about it floating around, many laboratories do not set compensation properly. (Mario Roederer)
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