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Organotypic Hippocampal Slice Cultures

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Protocol from Alpha MED Sciences Co., Ltd. Methods of organotypic slice cultures of brain tissue were originally developed in the roller tube (1) and on the semi-permeable membrane (2, 3) and applied sucessfully on the non-permeable multielectrode probe (4). 1. Gahwiler BH, Experientia, 40:253,1984 2. Yamamoto, N.et al., Science 245, 192-194., 1989 3. Stoppini, L., et al., J. Neurosci. Meth.37: 173, 1991 4. Shimono, K. et al., J. Neurosci. Meth.120: 192-202, 2002
 
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