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Protocol for Fluoro-Jade Histofluorescent Labeling of Degenerating Neurons

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Fluoro-Jade can be used on almost any tissue section. From our experience FJ will stain any tissue section type or thickness: i.e. frozen sections, vibratomed sections, cryostat sections, or paraffin embedded sections from 3-50 mm. The staining protocol takes approximately 90 minutes and little prior histological experience is necessary. When FJ-stained section are viewed under a fluorescence microscope with a FITC filter, degenerating neurons fluoresce brightly in contrast to neighboring neurons. (Neurotrauma Research Laboritories, UC Davis)
 
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