A novel method for constructing gene-targeting vectors
Abstract:
We developed a simple and rapid method for constructing knockout vectors using inverse-PCR (IPCR). The method consists of three steps: (i) digestion of a target bacterial artificial chromosome with several restriction enzymes (six-base cutters) followed b Nucleic Acids Research.2000:28(16):e77
Pharmaceutical Frontier Research Laboratories, Central Pharmaceutical Research Institute, Japan Toba
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