Oligonucleotide labeling methods. 3. Direct labeling of oligonucleotides employing a novel, non-nucl

Abstract:

Novel CE-phosphoramidite (7a-e) and CPG (8a, c, d, e) reagents have been prepared from a unique 2-aminobutyl-1,3-propanediol backbone. The reagents have been used to directly label oligonucleotides with fluorescein, acridine, and biotin via automated DNA synthesis. The versatile 2-aminobutyl-1,3-propanediol backbone allows for labeling at any position (5', internal, and 3') during solid phase oligonucleotide synthesis. Multiple labels can be achieved by repetitive coupling cycles. Furthermore, the 3-carbon atom internucleotide phosphate distance is retained when inserted internally. Using this method, individual oligonucleotides possessing two and three different reporter molecules have been prepared.

Nucleic Acids Research.1992:20(23):6253

Nucleic Acids Chemistry Division, Clontech Laboratories, Inc., Palo Alto, CA 94303.

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