biowww.net
Home /Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Pharmacology /Jobs /Books /Blog /Methods
Search methods database:

Top / Mutagenesis methods


Improved primer design for PCR-based, site-directed mutagenesis.



Improved primer design for PCR-based, site-directed mutagenesis.

Abstract:

Nucleic Acids Research.1992:20(5):1147

Max-Planck-Institut für Immunbiologie, Freiburg, FRG.

Report broken link | Email this to a friend.


Post Mutagenesis methods related comment or question in troubleshooting forum

Discussion and comment

By K.Sathish Kumar. on 17-Jan-2006
I am k.Sathish Kumar, Rightnow i am doing some site directed mutagesis for that i want to design some primers.How can i improve the primer design? and what are the parameters for SDM Primer designing?
Thank you.


Your Name
Your Email
Your Comment
Sponsored Links
Related papers
A new PCR based method for the generation of nested deletions.

A simple and rapid method for generating a deletion by PCR.

A novel rapid method for detection of PCR products.

Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% eff

An efficient one-step site-directed and site-saturation mutagenesis protocol

TAMS technology for simple and efficient

Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed an

A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR.

High efficiency of site-directed mutagenesis mediated by a single PCR product.

Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method.
About the site | Terms of service | Privacy policy | Ad Sponsorship | Contact us

Copyright 2000-2008 Biowww.net, All rights reserved