A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.
Abstract:
A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, subcloning is unnecessary and a high efficiency of mutation (58-97%) was obtained. If two synthetic oligonucleotide primers are used, two separate mutations can be simultaneously created in a single reaction tube. Nucleic Acids Research.1993:21(17):3977
Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, Piscata
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