Rapid Discrimination between Human Immunodeficiency Virus Type 2 Groups A and B by Real-Time PCR
Abstract:
We studied the feasibility of genotyping human immunodeficiency virus (HIV) type 2 groups A and B by real-time PCR. Two group-specific PCRs were developed. Real-time genotyping of 22 samples of genotype A, 10 samples of genotype B, and the isolate of new group H were compared to genotyping by sequencing and phylogeny. The group-specific PCRs specifically identified 84.3% of group A or B samples; isolate H was not detected. This method allowed rapid and specific discrimination between HIV-2 groups A and B and could be a useful tool for molecular epidemiological studies. Journal of Clinical Microbiology.2004:42(12):5866
Unité de Virologie, laboratoire associé au Centre National de Référence du VIH, Equipe d'Accueil
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