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Efficient Library Construction by In Vivo Recombination with a Telomere-Originated Autonomously Repl



Efficient Library Construction by In Vivo Recombination with a Telomere-Originated Autonomously Repl

Abstract:

A high frequency of transformation and an equal gene dosage between transformants are generally required for activity-based selection of mutants from a library obtained by directed evolution. An efficient library construction method was developed by using in vivo recombination in

Applied and Environmental Microbiology.2003:69(8):4448

Laboratory of Microbial Functions, Korea Research Institute of Bioscience and Biotechnology (KRIBB),

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