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Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of Infections by Influenza A  full-text paper
Abstract: Laboratory diagnosis of viral respiratory infections is generally performed by virus isolation in cell culture and immunofluorescent assays. Reverse transcriptase PCR is now recognized as a sensitive and specific alternative for detection of respiratory RNA vi ...

Comparison of the Real-Time PCR Method and the Gen-Probe Amplified  full-text paper
Abstract: Real-time PCR was compared to Amplified Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susce ...

A novel real-time quantitative PCR method using attached universal template probe  full-text paper
Abstract: A novel real-time quantitative polymerase chain reaction (PCR) method using an attached universal template (UT) probe is described. The UT is an approximately 20 base attachment to the 5�end of a PCR primer, and it can hybridize with a complementary TaqMan p ...

Real-time PCR-based method for the estimation of genome sizes  full-text paper
Abstract: The fast and reliable estimation of the genome sizes of various species would allow for a systematic analysis of many organisms and could reveal insights into evolutionary processes. Many methods for the estimation of genome sizes have already been described. ...

Real-Time PCR Method for Detection of  full-text paper
Abstract: The prevalence of microsporidiosis is likely underestimated due to the labor-intensive, insensitive, and nonspecific clinical laboratory methods used for the diagnosis of this disease. A real-time PCR assay was designed to assess DNA extraction methods and to ...

Sensitive and Specific Method for Rapid Identification of  full-text paper
Abstract: Molecular surveillance of pathogens has shown the need for rapid and dependable methods for the identification of organisms of clinical and epidemiological importance. As the leading cause of community-acquired pneumonia, was used as a model organism to devel ...

Efficient synthesis of double dye-labeled oligodeoxyribonucleotide probes and their application in a  full-text paper
Abstract: A fast cleaving non-nucleosidic tetramethylrhodamine dye-labeled support has been developed for automated synthesis of double dye-labeled oligodeoxyribonucleotides in high yield. A mixture (1:1:2) of t-butylamine:methanol:water is used for cleavage and deprote ...

Two Novel Real-Time Reverse Transcriptase PCR Assays for Rapid Detection of Bacterial Contamination  full-text paper
Abstract: The incidence of platelet bacterial contamination is approximately 1 per 2,000 units and has been acknowledged as the most frequent infectious risk from transfusion. In preliminary studies, the sterility of platelet concentrates (PCs) was tested with an automa ...

Development of a Real-Time PCR Assay for Rapid Detection and Quantification of  full-text paper
Abstract: The marine dinoflagellate genus includes a number of species which produce neurotoxins responsible for paralytic shellfish poisoning (PSP), which in humans may cause muscular paralysis, neurological symptoms, and, in extreme cases, death. is the most widespr ...

Rapid Discrimination between Human Immunodeficiency Virus Type 2 Groups A and B by Real-Time PCR  full-text paper
Abstract: We studied the feasibility of genotyping human immunodeficiency virus (HIV) type 2 groups A and B by real-time PCR. Two group-specific PCRs were developed. Real-time genotyping of 22 samples of genotype A, 10 samples of genotype B, and the isolate of new group ...

Dual-Probe Assay for Rapid Detection of Drug-Resistant  full-text paper
Abstract: Mutations in particular nucleotides of genes coding for drug targets or drug-converting enzymes lead to drug resistance in . For rapid detection of drug-resistant in clinical specimens, a simple and applicable method is needed. Eight TaqMan minor groove binde ...

A Novel Multiplex Real-Time PCR Assay for Rapid Typing of Major Staphylococcal Cassette Chromosome  full-text paper
Abstract: We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome Journal of Clinical Microbiology.2004:42(7):3309 ...

Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real-Time PCR  full-text paper
Abstract: Monitoring of hepatitis B virus (HBV) DNA in serum by molecular methods has become the standard for assessment of the replicative activity of HBV. Several molecular assays for the detection and quantification of HBV DNA have been described. However, they usual ...

Real-Time PCR Assay Using Fine-Needle Aspirates and Tissue Biopsy Specimens for Rapid Diagnosis of M  full-text paper
Abstract: A real-time PCR assay was developed to diagnose and identify the causative agents of suspected mycobacterial lymphadenitis. Primers and probes for the real-time PCR were designed on the basis of the internal transcribed spacer sequence, enabling the recognitio ...

New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples  full-text paper
Abstract: The application of a real-time quantitative PCR method (5�nuclease assay), based on the use of a probe labeled at its 5�end with a stable, fluorescent lanthanide chelate, for the quantification of human fecal bifidobacteria was evaluated. The specificities ...

20-Aug-2008 02:26 pm