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Patterned library analysis: A method for the quantitative assessment of hypotheses concerning the de  full-text paper
Abstract: Site-directed mutagenesis and combinatorial libraries are powerful tools for providing information about the relationship between protein sequence and structure. Here we report two extensions that expand the utility of combinatorial mutagenesis for the quantit ...

A method for global protein expression and antibody screening on high-density filters of an arrayed  full-text paper
Abstract: We have developed a technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing. A human fetal brain cDNA library was directionally cloned in a bacterial vector that allows IPTG-inducible expressio ...

A fast and sensitive method for the evaluation of binding of phage clones selected from a surface di  full-text paper
Abstract: Nucleic Acids Research.1994:22(3):545 ...

Identification of genes up-regulated in dedifferentiating Nicotania glauca pith tissue, using an imp  full-text paper
Abstract: Pith explants of Nicotiana glauca grown in vitro in synthetic medium supplemented with 2,4 dichlorophenoxyacetic acid (2, 4 D), are induced to dedifferentiate. Treatment with actinomycin D within the first 4-8 h of culture (but not later) is lethal and the exp ...

A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA.  full-text paper
Abstract: Nucleic Acids Research.1992:20(11):2900 ...

An improved method for the construction of high efficiency cDNA library in plasmid or lambda vector.  full-text paper
Abstract: Nucleic Acids Research.1990:18(4):1071 ...

Efficient Library Construction by In Vivo Recombination with a Telomere-Originated Autonomously Repl  full-text paper
Abstract: A high frequency of transformation and an equal gene dosage between transformants are generally required for activity-based selection of mutants from a library obtained by directed evolution. An efficient library construction method was developed by using in v ...

Efficient construction of a large nonimmune phage antibody library: The production of high-affinity  full-text paper
Abstract: A large library of phage-displayed human single-chain Fv antibodies (scFv), containing 6.7 × 10Proceedings of the National Academy of Sciences of the United States of America.1998:95(11):6157 ...

Selection of efficient cleavage sites in target RNAs by using a ribozyme expression library.  full-text paper
Abstract: Inactivation of gene expression by antisense mechanisms in general and by ribozymes in particular is a powerful technique for studying the function of a gene product. We have designed a strategy for expression of ribozymes, for selection of accessible cleavage ...

A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA c  full-text paper
Abstract: Nucleic Acids Research.1991:19(8):1954 ...

Synthesis of a highly efficient cDNA library.  full-text paper
Abstract: Nucleic Acids Research.1991:19(16):4559 ...

Functional cDNA library for efficient expression of measles virus-specific gene products in primate  full-text paper
Abstract: A cDNA library designed for high-level expression of measles virus-specific gene products in mammalian cells was generated. From this library, functional clones which contained the entire protein-coding sequences of the nucleocapsid (N) and the phosphoprotein ...

Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage an  full-text paper
Abstract: Peripheral blood leukocytes incubated with a semisynthetic phage antibody library and fluorochrome-labeled CD3 and CD20 antibodies were used to isolate human single-chain Fv antibodies specific for subsets of blood leukocytes by flow cytometry. Isolated phage ...

Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatogra  full-text paper
Abstract: Current methods for identifying mycobacteria by high-performance liquid chromatography (HPLC) require a visual assessment of the generated chromatographic data, which often involves time-consuming hand calculations and the use of flow charts. Our laboratory ha ...

A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA libr  full-text paper
Abstract: A procedure that uses the PCR to make rapid successive steps through a random-primed cDNA library has been developed to provide a method for sequencing very long genes that are difficult to obtain as a single clone. In each successive step, the portions of par ...

Rapid screening of a human genomic library in yeast artificial chromosomes for single-copy sequences  full-text paper
Abstract: A yeast artificial chromosome (YAC) library in Saccharomyces cerevisiae consisting of 30,000 clones with an average insert size of 0.1 megabase pair of human DNA has been generated from primary fibroblast DNA. A YAC vector was modified to enable the recovery o ...

Rapid plasmid library screening using RecA-coated biotinylated probes.  full-text paper
Abstract: A method for the rapid physical isolation of recombinant plasmids of interest from a mixture of plasmids such as a plasmid cDNA library is presented. This method utilizes the ability of RecA protein to form stable complexes between linear single-stranded and c ...

Construction of a human chromosome 3 specific NotI linking library using a novel cloning procedure.  full-text paper
Abstract: Two new diphasmid vectors (lambda SK17 and SK22) and a novel procedure to construct linking libraries are described. A partial filling-in reaction provides counter-selection against false linking clones in the library, and obviates the need for supF selection. ...

Construction of Long-Transcript Enriched cDNA Libraries from Submicrogram Amounts of Total RNAs by a  full-text paper
Abstract: [The sequence data described in this paper have been submitted to the GenBank data library under accession numbers Genome Research.2001:11(9):1553 ...

In vitro method for the generation of protein libraries using PCR amplification of a single DNA mole  full-text paper
Abstract: A novel in vitro method for the generation of a protein library has been developed using the polymerase chain reaction (PCR) amplification of a single DNA molecule followed by in vitro coupled transcription/translation. DNA template encoding green fluorescent ...

02-Dec-2008 03:13 pm