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Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments  full-text paper
Abstract: The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lox from the lox/Cre recombinase system of bacteriophage P1. There are two distinct stages. Firstly, vector and fragment DNAs are ligated intermolecularly under c ...

A method for efficient and rapid cloning of cDNA into lambda gt10 that facilitates directional sub-c  full-text paper
Abstract: Nucleic Acids Research.1991:19(8):1953 ...

A method for cloning and sequencing long palindromic DNA junctions  full-text paper
Abstract: DNA sequences containing long adjacent inverted repeats (palindromes) are inherently unstable and are associated with many types of chromosomal rearrangements. The instability associated with palindromic sequences also creates difficulties in their molecular a ...

Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme s  full-text paper
Abstract: We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation-hybridisat ...

A cloning method to identify caspases and their regulators in yeast: Identification of  full-text paper
Abstract: Site-specific proteases play critical roles in regulating many cellular processes. To identify novel site-specific proteases, their regulators, and substrates, we have designed a general reporter system in Proceedings of the National Academy of Sciences of ...

Large CAG/CTG repeat templates produced by PCR, usefulness for the DIRECT method of cloning genes wi  full-text paper
Abstract: We report here a simple method for generating large CAG/CTG repeat sequences. We have applied this method to clone the genomic sequence containing the CAG/CTG repeat and its upstream intronic sequence present in spinocerebellar ataxia type 3 or Machado-Joseph ...

A novel expression cloning method to isolate mammalian transcription factors in Schizosaccharomyces  full-text paper
Abstract: We describe a novel expression cloning strategy in the fission yeast for the isolation of mammalian transcription factors using a mammalian promoter as target. This strategy is possible because of the conservation between mammalian cells and Schizosaccharomyce ...

A method for directed evolution and functional cloning of enzymes  full-text paper
Abstract: A general scheme is described for the Proceedings of the National Academy of Sciences of the United States of America.1998:95(18):10523 ...

Development of an in vitro cloning method for Cowdria ruminantium.  full-text paper
Abstract: Cowdria ruminantium is a tick-borne rickettsia which causes severe disease in ruminants. All studies with C. ruminantium reported so far were carried out with stocks consisting of infective blood collected from reacting animals or from the same stocks propagat ...

Cloning (CAG/GTC)n STSs by an Alu-(CAG/GTC)n PCR method: an approach to human chromosome 12 and spin  full-text paper
Abstract: We report here an Alu-(CAG/GTC)n PCR method for the cloning of STSs with (CAG/GTC)n sequences. We have applied this method to genomic DNA of a somatic cell hybrid containing human chromosome 12 where linkage has been found for a known familiar dominant ataxia ...

A rapid and efficient method for the cloning of cosmid end-pieces.  full-text paper
Abstract: Nucleic Acids Research.1995:23(8):1441 ...

A recombinatorial method useful for cloning dominant alleles in Saccharomyces cerevisiae.  full-text paper
Abstract: Nucleic Acids Research.1995:23(3):537 ...

The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.  full-text paper
Abstract: A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E ...

Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of  full-text paper
Abstract: Nucleic Acids Research.1994:22(9):1764 ...

A rapid method for cloning mutagenic DNA repair genes: isolation of umu-complementing genes from mul  full-text paper
Abstract: Genetic and physiological experiments have demonstrated that the products of the umu-like operon are directly required for mutagenic DNA repair in enterobacteria. To date, five such operons have been cloned and studied at the molecular level. Given the apparen ...

Coincidence painting: a rapid method for cloning region specific DNA sequences.  full-text paper
Abstract: We have developed a novel coincidence cloning strategy, termed Coincidence Painting, which enables the rapid generation of large numbers of region specific sequences. Coincidence Painting utilises Degenerate Oligonucleotide Primed PCR (DOP-PCR) amplification o ...

A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction.  full-text paper
Abstract: An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacil ...

A method for cDNA cloning in COS cells irrespective of subcellular site of expression.  full-text paper
Abstract: Nucleic Acids Research.1991:19(16):4556 ...

A simple method for cloning blunt ended DNA fragments.  full-text paper
Abstract: Nucleic Acids Research.1991:19(2):398 ...

PCR driven DNA-DNA competitive hybridization: a new method for sensitive differential cloning.  full-text paper
Abstract: Nucleic Acids Research.1991:19(17):4778 ...

A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.  full-text paper
Abstract: Nucleic Acids Research.1991:19(5):1156 ...

General method for direct cloning of DNA fragments generated by the polymerase chain reaction.  full-text paper
Abstract: Nucleic Acids Research.1991:19(16):4560 ...

A highly efficient directional cDNA cloning method utilizing an asymmetrically tailed linker-primer  full-text paper
Abstract: A new procedure using an asymmetrically tailed linker-primer plasmid has been developed to prepare extremely high complexity cDNA libraries. This procedure yields plasmid primed libraries with a final form equivalent to those made by the procedure of Okayama a ...

A simple method for direct cloning cDNA sequence that flanks a region of known sequence from total R  full-text paper
Abstract: Nucleic Acids Research.1990:18(7):1922 ...

High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by  full-text paper
Abstract: We describe a highly efficient alkali cation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of fission yeast Schizosaccharomyces pombe mutants. cDNA libraries constructed with the pcD or pcD2 vector are transduced i ...

A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-ch  full-text paper
Abstract: We have devised a strategy based on polymerase chain reaction (PCR) for the rapid cloning of functional antibody genes as single-chain immunotoxins. RNA from a hybridoma producing an antibody (OVB3) that reacts with ovarian cancer cells was used as a template ...

A method for difference cloning: gene amplification following subtractive hybridization.  full-text paper
Abstract: We describe a procedure for genomic difference cloning, a method for isolating sequences present in one genomic DNA population ("tester") that is absent in another ("driver"). By subtractive hybridization, a large excess of driver is used to remove sequences c ...

A facile and reversible method to decrease the copy number of the ColE1-related cloning vectors comm  full-text paper
Abstract: We report a technique which uses the cointegrate intermediate of transposon Tn1000 transposition as a means to lower the copy number of ColE1-type plasmids. The transposition of Tn1000 from one replicon to another is considered a two-step process. In the first ...

A simple method for direct cloning and sequencing cDNA by the use of a single specific oligonucleoti  full-text paper
Abstract: Nucleic Acids Research.1989:17(1):453 ...

A method for the cloning of unpurified single-stranded oligonucleotides.  full-text paper
Abstract: Nucleic Acids Research.1989:17(17):7113 ...

Simple and efficient method for cloning of large DNA fragments with identical ends into plasmid vect  full-text paper
Abstract: Nucleic Acids Research.1988:16(9):4179 ...

Cloning the DdeI restriction-modification system using a two-step method.  full-text paper
Abstract: DdeI, a Type II restriction-modification system from the gram-negative anaerobic bacterium Desulfovibrio desulfuricans, recognizes the sequence CTNAG. The system has been cloned into E. coli in two steps. First the methylase gene was cloned into pBR322 and a d ...

General method for cloning Neurospora crassa nuclear genes by complementation of mutants.  full-text paper
Abstract: We have developed a sib selection procedure for cloning Neurospora crassa nuclear genes by complementation of mutants. This procedure takes advantage of a modified N. crassa transformation procedure that gives as many as 10,000 to 50,000 stable transformants p ...

Directional cloning of DNA fragments at a large distance from an initial probe: a circularization me  full-text paper
Abstract: The principle of a DNA cloning procedure that directionally generates genomic DNA fragments 50-2000 kilobases away from an initial probe is presented. The method depends on partial digestion of high molecular weight genomic DNA and subsequent ligation at very ...

General method for cloning amplified DNA by differential screening with genomic probes.  full-text paper
Abstract: Mutant Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate, a potent and specific inhibitor of aspartate transcarbamylase, have amplified the gene coding for the multifunctional protein (CAD) that includes this activity. The average amount of ...

A cloning method for caspase substrates that uses the yeast two-hybrid system: Cloning of the antiap  full-text paper
Abstract: Proceedings of the National Academy of Sciences of the United States of America.1998:95(15):8532 ...

Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments  full-text paper
Abstract: Nucleic Acids Research.1993:21(4):817 ...

A method for efficient and rapid cloning of cDNA into lambda gt10 that facilitates directional sub-c  full-text paper
Abstract: Nucleic Acids Research.1991:19(8):1953 ...

Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme s  full-text paper
Abstract: Nucleic Acids Research.1999:27(19):e26 ...

A cloning method to identify caspases and their regulators in yeast: Identification of  full-text paper
Abstract: Proceedings of the National Academy of Sciences of the United States of America.1999:96(6):2885 ...

Large CAG/CTG repeat templates produced by PCR, usefulness for the DIRECT method of cloning genes wi  full-text paper
Abstract: Nucleic Acids Research.1998:26(5):1352 ...

A novel expression cloning method to isolate mammalian transcription factors in Schizosaccharomyces  full-text paper
Abstract: Nucleic Acids Research.1998:26(22):5223 ...

A method for directed evolution and functional cloning of enzymes  full-text paper
Abstract: Proceedings of the National Academy of Sciences of the United States of America.1998:95(18):10523 ...

Development of an in vitro cloning method for Cowdria ruminantium.  full-text paper
Abstract: Clinical and Diagnostic Laboratory Immunology.1997:4(5):620 ...

Cloning (CAG/GTC)n STSs by an Alu-(CAG/GTC)n PCR method: an approach to human chromosome 12 and spin  full-text paper
Abstract: Nucleic Acids Research.1996:24(18):3651 ...

A rapid and efficient method for the cloning of cosmid end-pieces.  full-text paper
Abstract: Nucleic Acids Research.1995:23(8):1441 ...

A recombinatorial method useful for cloning dominant alleles in Saccharomyces cerevisiae.  full-text paper
Abstract: Nucleic Acids Research.1995:23(3):537 ...

The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.  full-text paper
Abstract: Nucleic Acids Research.1994:22(12):2399 ...

Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of  full-text paper
Abstract: Nucleic Acids Research.1994:22(9):1764 ...

A rapid method for cloning mutagenic DNA repair genes: isolation of umu-complementing genes from mul  full-text paper
Abstract: Journal of Bacteriology.1993:175(17):5411 ...

Coincidence painting: a rapid method for cloning region specific DNA sequences.  full-text paper
Abstract: Nucleic Acids Research.1993:21(22):5117 ...

A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction.  full-text paper
Abstract: Journal of Bacteriology.1992:174(15):5171 ...

07-Sep-2008 02:46 pm