Methods
Isolation of human immunodeficiency virus type 1 (HIV-1) RNA from feces by a simple method and diffe
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Abstract:
A simple method for the isolation and subsequent detection of human immunodeficiency virus type 1 (HIV-1) RNA from feces is described. Viral RNA was isolated by the method developed by Boom et al. (R. Boom, C.J.A. Sol, M.M.M. Salimans, C.L. Jansen, P.M.E. We ...
Simple method of RNA isolation from human leucocytic cell lines.
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Nucleic Acids Research.1993:21(20):4852 ...
A simple method for isolation of intact RNA from dried polyacrylamide gels.
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Nucleic Acids Research.1993:21(8):2016 ...
An efficient method for isolation of RNA and DNA from plants containing polyphenolics.
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Nucleic Acids Research.1992:20(9):2381 ...
An efficient method for isolation of RNA from tissue cultured plant cells.
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Nucleic Acids Research.1991:19(21):6055 ...
A rapid membrane-based viral RNA isolation method for the polymerase chain reaction.
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Nucleic Acids Research.1991:19(14):4011 ...
A high yield affinity purification method for specific RNA-binding proteins: isolation of the iron r
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We describe a simple method for the affinity purification of specific RNA-binding proteins. DNA sequences corresponding to the protein-binding site of the RNA are subcloned into an in vitro transcription vector between the T7 viral promoter and a poly(A) tra ...
RNA isolation: a mini-prep method.
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Abstract: Nucleic Acids Research.1988:16(22):10933 ...
A rapid and convenient method for isolation of nuclear, cytoplasmic and total cellular RNA.
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Abstract: Nucleic Acids Research.1988:16(22):10934 ...
Parvovirus RNA transcripts containing sequences not present in mature mRNA: a method for isolation o
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We report here a method of RNA preparation that may enrich for precursor RNA sequences and the results of an investigation of adeno-associated virus (AAV) RNA transcription that used this method. Whole cells were lysed with detergent and high salt and separa ...
Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.
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We present a rapid and efficient method for the detection of related transcripts with different expression levels. This approach combines the rapid amplification of cDNA ends (RACE) method with a cDNA subtractive technique. The strategy is based on successiv ...
Efficient Isolation of Human Immunodeficiency Virus Type 1 RNA from Cervical Swabs
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An efficient method for the isolation of human immunodeficiency virus type 1 (HIV-1) nucleic acids from dry cervical swabs was developed. HIV-1 Journal of Clinical Microbiology.1998:36(8):2349 ...
A universal and efficient protocol for the isolation of RNA from tissues and cultured cells.
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Abstract: Nucleic Acids Research.1988:16(5 Pt B):2340 ...
A rapid and convenient method for isolation of nuclear, cytoplasmic and total cellular RNA.
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Abstract: Nucleic Acids Research.1988:16(22):10934 ...
Automated Procedure for Improving the RNA Isolation Step in Viral Load Testing for Human Immunodefic
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An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantl ...
Detection of high levels of polyadenylate-containing RNA in bacteria by the use of a single-step RNA
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A new one-step procedure for the isolation of bacterial RNA, involving lysis by proteinase K in the presence of sodium dodecyl sulfate, is described. Pulse-labeled RNA isolated by this procedure for Bacillus brevis, Bacillus subtilis, and Escherichia coli B ...
New procedure for isolation of Rous sarcoma virus-specific RNA from infected cells.
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The use of mercurated "strong stop" complementary DNA (complementary to the 5'-terminal 101 nucleotides of Rous sarcoma virus RNA) in the isolation of virus-specific RNA from infected chicken embryo fibroblasts is described. Strong stop Rous sarcoma virus co ...
Purification of RNA and RNA-protein complexes by an R17 coat protein affinity method.
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We describe an affinity chromatography method to isolate specific RNAs and RNA-protein complexes formed in vivo or in vitro. It exploits the highly selective binding of the coat protein of bacteriophage R17 to a short hairpin in its genomic RNA. RNA containi ...
Preparative-scale purification of RNA using an efficient method which combines gel electrophoresis a
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Here we describe a reliable method for purifying large amounts of RNA of any sequence and length with comparable efficiency and resolution of gel electrophoresis and with capacity approaching that of column chromatography. The RNA mixture of interest is sepa ...
Sephadex-binding RNA ligands:
rapid affinity purification of RNA from complex RNA mixtures
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Sephadex-binding RNA ligands (aptamers) were obtained through Nucleic Acids Research.2000:29(2):e4 ...
Mono Q chromatography permits recycling of DNA template and purification of RNA transcripts after T7
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Nucleic Acids Research.1991:19(10):2786 ...
Reverse Sanger sequencing of RNA by MALDI-TOF mass spectrometry after solid phase purification
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Several DNA/RNA sequencing strategies have been developed using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). In the reverse Sanger sequencing approach α-thiophosphate-containing NTPs are employed. Sequencing ladde ...
Effective amplification of long targets from cloned inserts and human genomic DNA
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BMC Genomics.1994:91():5695 ...
Specific polyadenylation and purification of total messenger RNA from Escherichia coli.
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Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for want of a poly(A) tail on these messages. We have used poly(A) polymerase from yeast to effect specific polyadenylation of Escherichia coli polysomal mRNA in the pre ...
Synthesis, deprotection, analysis and purification of RNA and ribozymes.
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Improvements in the synthesis, deprotection and purification of oligoribonucleotides are described. These advances allow for reduced synthesis and deprotection times, while improving product yield. Coupling times are reduced by half using 5-ethylthio-1H-tetr ...
Synthesis of backbone deuterium labelled [r(CGCGAAUUCGCG)]2 and HPLC purification of synthetic RNA.
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The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2 (U* = [5'-2H]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60 degrees C. This procedure provided pure RNA dod ...
 06-Oct-2008 03:35 am
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