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Simple Chemical Extraction Method for DNA Isolation from  full-text paper
Abstract: DNA was successfully isolated from numerous Journal of Clinical Microbiology.2004:42(9):4293 ...

Evaluation of a New Automated, Standardized Generic Nucleic Acid Extraction Method (Total Nucleic Ac  full-text paper
Abstract: The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. T ...

Optimal DNA Isolation Method for Detection of Bacteria in Clinical Specimens by Broad-Range PCR  full-text paper
Abstract: Broad-range amplification of bacterial DNA from clinical specimens has proved useful for the diagnosis of various bacterial infections, especially during antimicrobial treatment of the patient. Optimal sample processing protocols for diagnostic broad-range b ...

PCR-Based Method for Isolation and Detection of  full-text paper
Abstract: Since current studies indicate the possible involvement of Clinical and Diagnostic Laboratory Immunology.2001:8(3):499 ...

A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers us  full-text paper
Abstract: Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by m ...

A rapid and gentle method for isolation of genomic DNA from pathogenic Nocardia spp.  full-text paper
Abstract: The lack of simple and efficient methods for extraction of DNA from Nocardia spp. has hampered molecular manipulation of the DNA for diagnostic purposes. In the present study, a method for the rapid extraction of undegraded genomic nocardial DNA was establis ...

A rapid method of DNA isolation using laundry detergent.  full-text paper
Abstract: Nucleic Acids Research.1996:24(8):1587 ...

A simple, rapid method for isolation of high quality genomic DNA from animal tissues.  full-text paper
Abstract: Nucleic Acids Research.1995:23(24):5087 ...

A rapid and simple method for the isolation of apoptotic DNA fragments.  full-text paper
Abstract: Nucleic Acids Research.1994:22(24):5506 ...

Midiprep method for isolation of DNA from plants with a high content of polyphenolics.  full-text paper
Abstract: Nucleic Acids Research.1993:21(14):3328 ...

A simple method for isolation of very high molecular weight DNA from Drosophila embryos.  full-text paper
Abstract: Nucleic Acids Research.1993:21(21):4985 ...

A rapid and gentle method for the isolation of genomic DNA from mycobacteria.  full-text paper
Abstract: Nucleic Acids Research.1993:21(10):2529 ...

A rapid method for the isolation of DNA-binding proteins from purified nuclei of tissues and cells i  full-text paper
Abstract: We describe a rapid and general method for isolating DNA-binding proteins in high yield from purified nuclei of animal cells. The method has been tested for the isolation of a series of different DNA-binding activities including those of transcription factor ...

An improved method for the isolation of chromosomal DNA from various bacteria and cyanobacteria.  full-text paper
Abstract: Nucleic Acids Research.1992:20(15):4101 ...

A rapid and inexpensive method for isolation of shuttle vector DNA from yeast for the transformation  full-text paper
Abstract: Nucleic Acids Research.1992:20(21):5852 ...

An efficient method for isolation of RNA and DNA from plants containing polyphenolics.  full-text paper
Abstract: Nucleic Acids Research.1992:20(9):2381 ...

A simple method for the isolation of chromosomal DNA from gram positive or acid-fast bacteria.  full-text paper
Abstract: Nucleic Acids Research.1991:19(8):1955 ...

A simple and efficient method for isolation of DNA fragments from agarose gel.  full-text paper
Abstract: Nucleic Acids Research.1991:19(23):6656 ...

One step 'miniprep' method for the isolation of plasmid DNA.  full-text paper
Abstract: Nucleic Acids Research.1991:19(10):2792 ...

Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex  full-text paper
Abstract: Current efforts to map the human genome are focused on individual chromosomes or smaller regions and frequently rely on the use of somatic cell hybrids. We report the application of the polymerase chain reaction to direct amplification of human DNA from hybr ...

Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex  full-text paper
Abstract: Current efforts to map the human genome are focused on individual chromosomes or smaller regions and frequently rely on the use of somatic cell hybrids. We report the application of the polymerase chain reaction to direct amplification of human DNA from hybr ...

A rapid and simple method for the isolation of high molecular weight cellular and chromosome-specifi  full-text paper
Abstract: Nucleic Acids Research.1987:15(2):859 ...

An improved method for the isolation of high yields of bacteriophage lambda DNA.  full-text paper
Abstract: Nucleic Acids Research.1986:14(22):9220 ...

Microscale method for rapid isolation of covalently closed circular plasmid DNA from group N strepto  full-text paper
Abstract: A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolati ...

A rapid method for the isolation of circular DNA using an aqueous two-phase partition system.  full-text paper
Abstract: A method of isolating circular plasmid DNA from cleared lysates of E. coli is described. Purification is achieved by virtue of the rapid re-annealing kinetics or supercoiled DNA. After a brief denaturation step, double stranded plasmid DNA is separated from ...

Application of the avidin-biotin method of gene enrichment to the isolation of long double-stranded  full-text paper
Abstract: A method of enriching for long double-stranded segments of eukaryotic DNA carrying particular genes is described. A purified RNA coded for by the gene is covalently attached to biotin via the protein, cytochrome c. This modified RNA is hybridized to total nu ...

A method for the isolation of cross-linked nucleosides from DNA: application to cross-links induced  full-text paper
Abstract: A procedure is reported for the isolation of cross-linked nucleosides from nitrous acid-treated calf thymus DNA. Cross-linked DNA was hydrolyzed enzymatically with deoxyribonuclease I and snake venom phosphodiesterase and fractionated on a DEAE-Sephadex colu ...

A general method for isolation of high molecular weight DNA from eukaryotes.  full-text paper
Abstract: A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Cr ...

A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the clos  full-text paper
Abstract: Proceedings of the National Academy of Sciences of the United States of America.1967:57(5):1514 ...

A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, f  full-text paper
Abstract: Nucleic Acids Research.1992:20(22):6115 ...

Efficient DNA isolation within a single gel barrier tube.  full-text paper
Abstract: Nucleic Acids Research.1989:17(20):8393 ...

A rapid and efficient 'miniprep' for isolation of plasmid DNA.  full-text paper
Abstract: Nucleic Acids Research.1989:17(9):3604 ...

A simple and efficient non-organic procedure for the isolation of genomic DNA from blood.  full-text paper
Abstract: Nucleic Acids Research.1989:17(20):8390 ...

Improved Silica-Guanidiniumthiocyanate DNA Isolation Procedure Based on Selective Binding of Bovine  full-text paper
Abstract: DNA purified from clinical cerebrospinal fluid and urine specimens by a silica-guanidiniumthiocyanate procedure frequently contained an inhibitor(s) of DNA-processing enzymes which may have been introduced by the purification procedure itself. Inhibition cou ...

Combined subtraction hybridization and polymerase chain reaction amplification procedure for isolati  full-text paper
Abstract: A novel subtraction hybridization procedure, incorporating a combination of four separation strategies, was developed to isolate unique DNA sequences from a strain of Rhizobium leguminosarum bv. trifolii. Sau3A-digested DNA from this strain, i.e., the probe ...

Simplified mammalian DNA isolation procedure.  full-text paper
Abstract: Nucleic Acids Research.1991:19(15):4293 ...

A simple and economical procedure for large-scale plasmid DNA isolation.  full-text paper
Abstract: Nucleic Acids Research.1991:19(9):2507 ...

Simple procedure of DNA isolation from human serum.  full-text paper
Abstract: Nucleic Acids Research.1991:19(20):5792 ...

A rapid DNA isolation procedure from petri dish grown clinical bacterial isolates.  full-text paper
Abstract: Nucleic Acids Research.1990:18(21):6462 ...

A simple and efficient non-organic procedure for the isolation of genomic DNA from blood.  full-text paper
Abstract: Nucleic Acids Research.1989:17(20):8390 ...

A Sephadex column procedure for DNA isolation is also useful for detecting dsRNA.  full-text paper
Abstract: Nucleic Acids Research.1988:16(2):776 ...

Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis.  full-text paper
Abstract: A rapid and simple plasmid isolation procedure was developed for the epidemiological analysis of plasmid-mediated antimicrobial resistance. By this method, plasmid DNAs ranging in molecular weight between 2.0 and 122 X 10(6) could be detected. Various bacter ...

Direct selection procedure for the isolation of functional centromeric DNA.  full-text paper
Abstract: Several plasmids, each containing a segment of DNA capable of conferring mitotic stability on the ars1 vector YRp7 replicating in yeast (Saccharomyces cerevisiae), have been isolated from a yeast genomic library by direct selection for stable plasmid mainten ...

An improved method for the purification of high molecular weight bacterial chromosomal DNA.  full-text paper
Abstract: Nucleic Acids Research.1989:17(13):5405 ...

Modified method for double stranded DNA sequencing and synthetic oligonucleotide purification.  full-text paper
Abstract: Nucleic Acids Research.1988:16(21):10382 ...

A rapid method for the purification of DNA from blood.  full-text paper
Abstract: Nucleic Acids Research.1987:15(22):9611 ...

Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput autom  full-text paper
Abstract: Nucleic Acids Research.1995:23(23):4930 ...

Large scale chemical synthesis, purification and crystallization of RNA-DNA chimeras.  full-text paper
Abstract: RNA-DNA chimeras, in which both DNA and RNA monomers are site-specifically substituted in the same strand, may be prepared only by chemical synthesis. Biochemical studies have revealed a number of surprising and subtle effects resulting from the insertion of ...

Sequence-specific DNA purification by triplex affinity capture.  full-text paper
Abstract: A DNA isolation procedure was developed by using triple-helix formation and magnetic separation. In this procedure, target DNA is captured by a biotinylated oligonucleotide via intermolecular triplex formation, bound to streptavidin-coated magnetic beads, an ...

A fast procedure for yeast DNA purification.  full-text paper
Abstract: Nucleic Acids Research.1991:19(19):5443 ...

Gel electrophoresis using a fluorescence agent for analysis and purification of non-labeled syntheti  full-text paper
Abstract: Nucleic Acids Research.1991:19(14):4003 ...

Modified method for double stranded DNA sequencing and synthetic oligonucleotide purification.  full-text paper
Abstract: Nucleic Acids Research.1988:16(21):10382 ...

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