Methods
Sequence-Based Design of Single-Copy Genomic DNA Probes for Fluorescence In Situ Hybridization
full-text paper
Abstract: Chromosomal rearrangements are frequently monitored by fluorescence in situ hybridization (FISH) using large, recombinant DNA probes consisting of contiguous genomic intervals that are often distant from disease loci. We developed smaller, targeted, single-cop ...
Alkaline fixation drastically improves the signal of
full-text paper
Abstract:
Nucleic Acids Research.2000:28(10):e46 ...
Differentially Painting Human Chromosome Arms with Combined Binary Ratio-labeling Fluorescence In Si
full-text paper
Abstract: Recently we developed a novel strategy for differentially painting all 24 human chromosomes. It is termed COBRA–FISH, short for combined binary ratio labeling–fluorescence in situ hybridization. COBRA–FISH is distinct from the pure comb ...
Telomere analysis by fluorescence in situ hybridization and flow cytometry.
full-text paper
Abstract: Determination of telomere length is traditionally performed by Southern blotting and densitometry, giving a mean telomere restriction fragment (TRF) value for the total cell population studied. Fluorescence in situ hybridization (FISH) of telomere repeats has ...
Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and h
full-text paper
Abstract: A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. Th ...
High-resolution mapping of mammalian genes by in situ hybridization to free chromatin.
full-text paper
Abstract: Fluorescence in situ hybridization to metaphase chromosomes or chromatin fibers in interphase nuclei is a powerful technique in mapping genes and DNA segments to specific chromosome region. We have been able to release the chromatin fibers from cells arrested ...
In situ hybridization with fluoresceinated DNA.
full-text paper
Abstract: We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The fea ...
Exonuclease digestion of human chromosomes for in situ hybridization and R-banding.
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Abstract: Nucleic Acids Research.1990:18(9):2831 ...
Mechanistic Approach to the Problem of Hybridization Efficiency in Fluorescent In Situ Hybridization
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Abstract: In fluorescent in situ hybridization (FISH), the efficiency of hybridization between the DNA probe and the rRNA has been related to the accessibility of the rRNA when ribosome content and cell permeability are not limiting. Published rRNA accessibility maps sh ...
 07-Oct-2008 11:16 am
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