Methods
A novel method for constructing gene-targeting vectors
full-text paper
Abstract: We developed a simple and rapid method for constructing knockout vectors using inverse-PCR (IPCR). The method consists of three steps: (i) digestion of a target bacterial artificial chromosome with several restriction enzymes (six-base cutters) followed bNu ...
A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
full-text paper
Abstract: Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.749203.Genome Research.2002:13(3):476 ...
A simplified method of generating transgenic Xenopus
full-text paper
Abstract: Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has tNu ...
A method for the generation of YAC transgenic mice by pronuclear microinjection.
full-text paper
Abstract: Yeast artificial chromosomes (YACs) represent the latest generation of vectors which have the great advantage of large insert size. The introduction of YACs into mammalian cells and organisms has become an important goal, since it offers the potential toNuc ...
A rapid and economical method for screening transgenic mice.
full-text paper
Abstract: Nucleic Acids Research.1989:17(9):3608 ...
A New Method for Rapidly Generating Gene-Targeting Vectors by Engineering BACs Through Homologous Recombination in Bacteria
full-text paper
Abstract: Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.1356503. Article published online before print in August 2003.Genome Research.2003:13(9):2190 ...
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