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Novel Method of mRNA Degradation
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Abstract: RNase mitochondrial RNA processing (RNase MRP) mutants have been shown to have an exit-from-mitosis defect that is caused by an increase in Molecular and Cellular Biology.2003:24(3):945 ...
A rapid in vitro method for obtaining RNA accessibility patterns for complementary DNA probes: corre
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Abstract: A technique is described to identify the rare sequences within an RNA molecule that are available for efficient interaction with complementary DNA probes: the target RNA is digested by RNase H in the presence of a random pool of complementary DNA fragments gen ...
Method for determining RNA 3' ends and application to human telomerase RNA.
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Abstract: Nucleic Acids Research.1996:24(3):532 ...
A comparative method for finding and folding RNA secondary structures within protein-coding regions
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Abstract: Existing computational methods for RNA secondary-structure prediction tacitly assume RNA to only encode functional RNA structures. However, experimental studies have revealed that some RNA sequences, e.g. compact viral genomes, can simultaneously encode functi ...
RNA canonical and non-canonical base pairing types: a recognition method and complete repertoire
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Abstract: The problem of systematic and objective identification of canonical and non-canonical base pairs in RNA three-dimensional (3D) structures was studied. A probabilistic approach was applied, and an algorithm and its implementation in a computer program that dete ...
Selective detection of ribose-methylated nucleotides in RNA by a mass spectrometry-based method.
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Abstract: Post-transcriptional methylation of ribose at position O-2' is one of the most common and conserved types of RNA modification. Details of the functional roles of these methylations are far from clear, although in tRNA they are involved at position 34 in regula ...
Transcriptional sequencing: A method for DNA sequencing using RNA polymerase
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Abstract: We have developed a sequencing method based on the RNA polymerase chain termination reaction with rhodamine dye attached to 3′-deoxynucleoside triphosphate (3′-dNTP). This method enables us to conduct a rapid isothermal sequencing reaction in & ...
A new method to monitor the rate of conformational transitions in RNA.
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Abstract: Many RNAs need Mg2+to produce stable tertiary structures. Here we describe a simple method to measure the rate and activation parameters of tertiary structure unfolding that exploits this Mg2+dependence. Our approach is based on mixing an RNA solution with exc ...
Rapid purification of RNA secondary structures
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Abstract: A new method for rapid purification and structural analysis of oligoribonucleotides of 19 and 20 nt is applied to RNA hairpins SL3 and SL2, which are stable secondary structures present on the ψ recognition element of HIV-1. This approach uses ion-pair ...
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14-Sep-2009 01:53 pm
