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Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids c  full-text paper
Abstract: A simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described. This in vitro transcription system is based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcript ...

Analysis of HLA-D micropolymorphism by a simple procedure: RNA oligonucleotide hybridization.  full-text paper
Abstract: Recent progress in the molecular genetics of HLA class II antigens has revealed the existence of multiple loci and of a large degree of polymorphism, with more individual alleles than was expected. An accurate detection and analysis of this extensive polymorph ...

A homogeneous method to quantify mRNA levels: a hybridization of RNase protection and scintillation  full-text paper
Abstract: A novel method to measure mRNA levels has been developed by combining the detection capabilities of RNase protection (RPA) with the quantification advantages of scintillation proximity assay (SPA) technology. Sample processing is reduced to the addition of a s ...

Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization sy  full-text paper
Abstract: We report a novel procedure to construct a normalized (equalized) cDNA library. By introduction of the highly efficient self-hybridization system between a whole mRNA population and their corresponding cDNA immobilized on latex beads, which involves relatively ...

Dot blot hybridization using cytoplasmic extracts is inappropriate for determination of mRNA levels  full-text paper
Abstract: Nucleic Acids Research.1990:18(2):382 ...

An analysis of the rate of metallothionein mRNA poly(A)-shortening using RNA blot hybridization.  full-text paper
Abstract: A progressive reduction in the size of rat metallothionein-1 mRNA following induction by copper chloride or dexamethasone was demonstrated on RNA blots, and was shown to be due to shortening of the poly(A)-tail. The rate of poly(A) removal was the same in rat ...

Strength of translation initiation signal sequence of mRNA as studied by quantification method: effe  full-text paper
Abstract: Concerning the translation initiation signals in vertebrate mRNAs, both the ATG initiation codon and the sequences flanking the initiation codon are required to direct the position of initiation. A consensus sequence for the signal, (GCC)GCC(A or G)CCATGG, has ...

A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA le  full-text paper
Abstract: A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy ( ...

Prioritized selection of oligodeoxyribonucleotide probes for efficient hybridization to RNA transcri  full-text paper
Abstract: Only a small fraction of short oligonucleotide probes bind efficiently to complementary segments in long RNA transcripts. Technologies such as array-based transcript profiling and antisense control of gene expression would benefit greatly from a method for pre ...

07-Oct-2008 01:23 pm