Methods
A novel method for determining linkage between DNA sequences: hybridization to paired probe arrays.
full-text paper
Abstract: Cooperative hybridization has been used to establish physical linkage between two loci on a DNA strand. Linkage was detected by hybridization to a new type of high-density oligonucleotide array. Each synthesis location on the array contains a mixture of two di ...
A simple and efficient enzymatic method for covalent attachment of DNA to cellulose. Application for
full-text paper
Abstract: Single-stranded DNAs (ssDNAs) were covalently bound by a simple and efficient enzymatic method to a solid support matrix and used to develop several new procedures for gene analysis. The novel procedure to prepare a ssDNA stably coupled to a solid support empl ...
Isolation of differentially expressed genes upon immunoglobulin class switching by a subtractive hyb
full-text paper
Abstract: Immunoglobulin class switch recombination enables B lymphocytes to sequentially express antibodies that have identical specificities but that differ in class and effector function. Although several cis elements required for class switch recombination have been ...
DNA sequencing by hybridization: 100 bases read by a non-gel-based method.
full-text paper
Abstract: Determination of the sequences of human and other complex genomes requires much faster and less expensive sequencing processes than the methods in use today. Sequencing by hybridization is potentially such a process. In this paper we present hybridization data ...
A sensitive, bioluminescent-enhanced detection method for DNA dot-hybridization.
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Abstract: Nucleic Acids Research.1988:16(3):1213 ...
A colorimetric method for DNA hybridization.
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Abstract: A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxi ...
Rapid and simple DNA detection by hybridization on absorbent filters.
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Abstract: Nucleic Acids Research.1991:19(6):1351 ...
Rapid quantitation of DNA spot hybridization by flatbed scintillation counting.
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Abstract: The flatbed scintillation counting system (Betaplate) was used for quantitative measurement of the radioactive hybridization signal in detection of adenovirus and papillomavirus DNA in clinical specimens. In this method, 96 samples on a nylon membrane can be h ...
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid
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Abstract: We describe a technique for transferring electrophoretically separated bands of double-stranded DNA from agarose gels to diazobenzyloxymethyl-paper. Controlled cleavage of the DNA in situ by sequential treatment with dilute acid, which causes partial depurinat ...
DNA purification and isolation using a solid-phase.
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Abstract: Nucleic Acids Research.1994:22(21):4543 ...
Purification of genomic DNA using heparin to remove nuclear proteins.
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Abstract: Nucleic Acids Research.1993:21(12):2952 ...
DNA Enrichment by Allele-Specific Hybridization (DEASH): A Novel Method for Haplotyping and for Dete
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Abstract: Detecting rare sequence variants in genomic DNA is central to the analysis of de novo mutation and recombination events and the detection of rare pathological mutations in mixed cell populations. Current PCR techniques suffer from noise that limits detection t ...
 02-Dec-2008 12:49 pm
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