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Methods

A simple and rapid method for generating a deletion by PCR.  full-text paper
Abstract: Nucleic Acids Research.1991:19(10):2785 ...

A novel rapid method for detection of PCR products.  full-text paper
Abstract: Nucleic Acids Research.1991:19(14):4012 ...

Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% eff  full-text paper
Abstract: Site-directed, Ligase-Independent Mutagenesis (SLIM) is a novel PCR-mediated mutagenesis approach that can accommodate all three sequence modification types (insertion, deletion and substitution). The method utilizes an inverse PCR amplification of the templat ...

An efficient one-step site-directed and site-saturation mutagenesis protocol  full-text paper
Abstract: We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of prime ...

TAMS technology for simple and efficient  full-text paper
Abstract: Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. However, most current protocols require the target DNA to be cloned into a plasmid vector before mutagenesis can be performed, and none of them are effective for mu ...

Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed an  full-text paper
Abstract: Nucleic Acids Research.2000:28(16):e78 ...

A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR.  full-text paper
Abstract: A rapid method is described to efficiently perform site-directed mutagenesis based on overlap extension polymerase chain reaction (OE-PCR). Two template DNA molecules in different orientations relative to only one universal primer were amplified in parallel. B ...

High efficiency of site-directed mutagenesis mediated by a single PCR product.  full-text paper
Abstract: We describe a highly efficient procedure for site-specific mutagenesis of double-stranded plasmids. The method relies on a single PCR primer which incorporates both the mutations at the selection site and the desired single base substitutions at the mutant sit ...

Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method.  full-text paper
Abstract: We describe a rapid and efficient megaprimer PCR procedure for site-directed mutagenesis that does not require any intermediate purification of DNA between the two rounds of PCR. This protocol is based on the design of forward and reverse flanking primers with ...

Rapid site-directed mutagenesis by a method that selects for full length mutated DNA.  full-text paper
Abstract: Nucleic Acids Research.1996:24(7):1378 ...

Recent advances in gene mutagenesis by site-directed recombination.  full-text paper
Abstract: Journal of Clinical Investigation.1996:97(9):1999 ...

Rapid and high efficiency site-directed mutagenesis by improvement of the homologous recombination t  full-text paper
Abstract: Nucleic Acids Research.1995:23(9):1642 ...

Improved method for PCR-mediated site-directed mutagenesis.  full-text paper
Abstract: Nucleic Acids Research.1994:22(3):541 ...

An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules.  full-text paper
Abstract: Nucleic Acids Research.1993:21(9):2277 ...

A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.  full-text paper
Abstract: A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, sub ...

Use of oligonucleotides and nick translation for site-directed mutagenesis in plasmids.  full-text paper
Abstract: Nucleic Acids Research.1992:20(4):922 ...

Improved primer design for PCR-based, site-directed mutagenesis.  full-text paper
Abstract: Nucleic Acids Research.1992:20(5):1147 ...

A simple in vitro site directed mutagenesis of concatamerized cDNA by inverse polymerase chain react  full-text paper
Abstract: Nucleic Acids Research.1992:20(19):5241 ...

Improved site-directed mutagenesis method using PCR.  full-text paper
Abstract: Nucleic Acids Research.1991:19(16):4558 ...

Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer.  full-text paper
Abstract: Nucleic Acids Research.1990:18(16):4947 ...

Site-directed, recombination-mediated mutagenesis of a complex gene locus.  full-text paper
Abstract: We have generated a site-specific 17 bp insertion within a 38 kb chick globin gene cluster by employing the recombination abilities of Saccharomyces cerevisiae. This gene cluster contains four beta-type globin genes which share a high degree of sequence homolo ...

Single step large scale site-directed in vitro mutagenesis using multiple oligonucleotides.  full-text paper
Abstract: Nucleic Acids Research.1990:18(24):7457 ...

PCR-based site-directed mutagenesis using primers with mismatched 3'-ends.  full-text paper
Abstract: Nucleic Acids Research.1990:18(10):3077 ...

Efficient site directed in vitro mutagenesis using ampicillin selection.  full-text paper
Abstract: A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer whic ...

The use of native T7 DNA polymerase for site-directed mutagenesis.  full-text paper
Abstract: Nucleic Acids Research.1989:17(13):5408 ...

Method to protect a targeted amino acid residue during random mutagenesis  full-text paper
Abstract: To generate a random mutant library that is free from mutation at a particular amino acid residue, we replace the codon of interest with a detachable, short DNA sequence containing a BsaXI recognition site. After PCR mutagenesis, this sequence is removed and i ...

Codon cassette mutagenesis: a general method to insert or replace individual codons by using univers  full-text paper
Abstract: We describe codon cassette mutagenesis, a simple method of mutagenesis that uses universal mutagenic cassettes to deposit single codons at specific sites in double-stranded DNA. A target molecule is first constructed that contains a blunt, double-strand break ...

A novel method to identify nucleic acid binding sites in proteins by scanning mutagenesis: applicati  full-text paper
Abstract: We describe a new procedure to identify RNA or DNA binding sites in proteins, based on a combination of UV cross-linking and single-hit chemical peptide cleavage. Site-directed mutagenesis is used to create a series of mutants with single Asn-Gly sequences in ...

Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.  full-text paper
Abstract: Nucleic Acids Research.1993:21(3):777 ...

An efficient method for isolation of promoter mutations after oligonucleotide-directed mutagenesis.  full-text paper
Abstract: Nucleic Acids Research.1990:18(17):5323 ...

Phasduction--a simplified protocol for oligonucleotide-directed mutagenesis by the gapped duplex DNA  full-text paper
Abstract: Nucleic Acids Research.1989:17(14):5862 ...

A simple and efficient method for chemical mutagenesis of DNA.  full-text paper
Abstract: A simple and efficient procedure for the generation of random GC to AT transition mutations in a specific DNA segment is described. A restriction fragment is inserted in each orientation into an M13 vector, single-stranded virion DNA from each recombinant phag ...

An efficient protocol for linker scanning mutagenesis: analysis of the translational regulation of a  full-text paper
Abstract: A protocol has been developed that is capable of saturating regions hundreds of basepairs in length with linker scanning mutations. The efficacy of this method stems from the design of the linker scanning mutagenesis (LSM) cassette which is composed of a selec ...

Three-step PCR mutagenesis for 'linker scanning'.  full-text paper
Abstract: 'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made wi ...

21-Aug-2008 07:14 pm