http://biowww.net/ Tue, 02 Dec 2008 05:43:11 +0100 FeedCreator 1.7.2 http://biowww.net/methods/detail-82.html Caspase-mediated proteolysis is a critical and central element of the apoptotic process; therefore, it is important to identify the downstream molecular targets of caspases. We established a method for cloning the genes of caspase substrates by two major modif ... TsujimotoYoshihide http://biowww.net/methods/detail-83.html The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lox from the lox/Cre recombinase system of bacteriophage P1. There are two distinct stages. Firstly, vector and fragment DNAs are ligated intermolecularly under c ... BoydA C http://biowww.net/methods/detail-84.html Nucleic Acids Research.1991:19(8):1953 ... TirindelliR http://biowww.net/methods/detail-85.html DNA sequences containing long adjacent inverted repeats (palindromes) are inherently unstable and are associated with many types of chromosomal rearrangements. The instability associated with palindromic sequences also creates difficulties in their molecular a ... RattrayAlison J. http://biowww.net/methods/detail-86.html We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation-hybridisat ... NeilanB A http://biowww.net/methods/detail-87.html Site-specific proteases play critical roles in regulating many cellular processes. To identify novel site-specific proteases, their regulators, and substrates, we have designed a general reporter system in Proceedings of the National Academy of Sciences of ... HayBruce A. http://biowww.net/methods/detail-88.html We report here a simple method for generating large CAG/CTG repeat sequences. We have applied this method to clone the genomic sequence containing the CAG/CTG repeat and its upstream intronic sequence present in spinocerebellar ataxia type 3 or Machado-Joseph ... EstivillX http://biowww.net/methods/detail-89.html We describe a novel expression cloning strategy in the fission yeast for the isolation of mammalian transcription factors using a mammalian promoter as target. This strategy is possible because of the conservation between mammalian cells and Schizosaccharomyce ... HuylebroeckD http://biowww.net/methods/detail-90.html A general scheme is described for the Proceedings of the National Academy of Sciences of the United States of America.1998:95(18):10523 ... SchultzPeter G. http://biowww.net/methods/detail-91.html Cowdria ruminantium is a tick-borne rickettsia which causes severe disease in ruminants. All studies with C. ruminantium reported so far were carried out with stocks consisting of infective blood collected from reacting animals or from the same stocks propagat ... BensaidA http://biowww.net/methods/detail-92.html We report here an Alu-(CAG/GTC)n PCR method for the cloning of STSs with (CAG/GTC)n sequences. We have applied this method to genomic DNA of a somatic cell hybrid containing human chromosome 12 where linkage has been found for a known familiar dominant ataxia ... EstivillX http://biowww.net/methods/detail-93.html Nucleic Acids Research.1995:23(8):1441 ... MüllerU http://biowww.net/methods/detail-94.html Nucleic Acids Research.1995:23(3):537 ... ThireosG http://biowww.net/methods/detail-95.html A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E ... XuS Y http://biowww.net/methods/detail-96.html Nucleic Acids Research.1994:22(9):1764 ... KarikóK http://biowww.net/methods/detail-97.html Genetic and physiological experiments have demonstrated that the products of the umu-like operon are directly required for mutagenic DNA repair in enterobacteria. To date, five such operons have been cloned and studied at the molecular level. Given the apparen ... WoodgateR http://biowww.net/methods/detail-98.html We have developed a novel coincidence cloning strategy, termed Coincidence Painting, which enables the rapid generation of large numbers of region specific sequences. Coincidence Painting utilises Degenerate Oligonucleotide Primed PCR (DOP-PCR) amplification o ... Ferguson-SmithM A http://biowww.net/methods/detail-99.html An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacil ... GrussA http://biowww.net/methods/detail-100.html Nucleic Acids Research.1991:19(16):4556 ... CleversH C http://biowww.net/methods/detail-101.html Nucleic Acids Research.1991:19(2):398 ... StuartK D http://biowww.net/methods/detail-102.html Nucleic Acids Research.1991:19(17):4778 ... CatsicasS http://biowww.net/methods/detail-103.html Nucleic Acids Research.1991:19(5):1156 ... GrahamM W http://biowww.net/methods/detail-104.html Nucleic Acids Research.1991:19(16):4560 ... WeisblumB http://biowww.net/methods/detail-105.html A new procedure using an asymmetrically tailed linker-primer plasmid has been developed to prepare extremely high complexity cDNA libraries. This procedure yields plasmid primed libraries with a final form equivalent to those made by the procedure of Okayama a ... MargolskeeR F http://biowww.net/methods/detail-106.html Nucleic Acids Research.1990:18(7):1922 ... HolcenbergJ http://biowww.net/methods/detail-107.html We describe a highly efficient alkali cation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of fission yeast Schizosaccharomyces pombe mutants. cDNA libraries constructed with the pcD or pcD2 vector are transduced i ... OkayamaH http://biowww.net/methods/detail-108.html We have devised a strategy based on polymerase chain reaction (PCR) for the rapid cloning of functional antibody genes as single-chain immunotoxins. RNA from a hybridoma producing an antibody (OVB3) that reacts with ovarian cancer cells was used as a template ... PastanI http://biowww.net/methods/detail-109.html We describe a procedure for genomic difference cloning, a method for isolating sequences present in one genomic DNA population ("tester") that is absent in another ("driver"). By subtractive hybridization, a large excess of driver is used to remove sequences c ... WiglerM http://biowww.net/methods/detail-110.html We report a technique which uses the cointegrate intermediate of transposon Tn1000 transposition as a means to lower the copy number of ColE1-type plasmids. The transposition of Tn1000 from one replicon to another is considered a two-step process. In the first ... CronanJ E http://biowww.net/methods/detail-111.html Nucleic Acids Research.1989:17(1):453 ... BerchtoldM W http://biowww.net/methods/detail-112.html Nucleic Acids Research.1989:17(17):7113 ... ScheragaH A http://biowww.net/methods/detail-113.html Nucleic Acids Research.1988:16(9):4179 ... TrávnícekM http://biowww.net/methods/detail-114.html DdeI, a Type II restriction-modification system from the gram-negative anaerobic bacterium Desulfovibrio desulfuricans, recognizes the sequence CTNAG. The system has been cloned into E. coli in two steps. First the methylase gene was cloned into pBR322 and a d ... BrooksJ E http://biowww.net/methods/detail-115.html We have developed a sib selection procedure for cloning Neurospora crassa nuclear genes by complementation of mutants. This procedure takes advantage of a modified N. crassa transformation procedure that gives as many as 10,000 to 50,000 stable transformants p ... LambowitzA M http://biowww.net/methods/detail-116.html The principle of a DNA cloning procedure that directionally generates genomic DNA fragments 50-2000 kilobases away from an initial probe is presented. The method depends on partial digestion of high molecular weight genomic DNA and subsequent ligation at very ... WeissmanS M http://biowww.net/methods/detail-117.html Mutant Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate, a potent and specific inhibitor of aspartate transcarbamylase, have amplified the gene coding for the multifunctional protein (CAD) that includes this activity. The average amount of ... StarkG R http://biowww.net/methods/detail-118.html Proceedings of the National Academy of Sciences of the United States of America.1998:95(15):8532 ... TsujimotoYoshihide http://biowww.net/methods/detail-119.html Nucleic Acids Research.1993:21(4):817 ... BoydA C http://biowww.net/methods/detail-120.html Nucleic Acids Research.1991:19(8):1953 ... TirindelliR http://biowww.net/methods/detail-122.html Nucleic Acids Research.1999:27(19):e26 ... NeilanB A http://biowww.net/methods/detail-123.html Proceedings of the National Academy of Sciences of the United States of America.1999:96(6):2885 ... HayBruce A. http://biowww.net/methods/detail-124.html Nucleic Acids Research.1998:26(5):1352 ... EstivillX http://biowww.net/methods/detail-125.html Nucleic Acids Research.1998:26(22):5223 ... HuylebroeckD http://biowww.net/methods/detail-126.html Proceedings of the National Academy of Sciences of the United States of America.1998:95(18):10523 ... SchultzPeter G. http://biowww.net/methods/detail-127.html Clinical and Diagnostic Laboratory Immunology.1997:4(5):620 ... BensaidA http://biowww.net/methods/detail-128.html Nucleic Acids Research.1996:24(18):3651 ... EstivillX http://biowww.net/methods/detail-129.html Nucleic Acids Research.1995:23(8):1441 ... MüllerU http://biowww.net/methods/detail-130.html Nucleic Acids Research.1995:23(3):537 ... ThireosG http://biowww.net/methods/detail-131.html Nucleic Acids Research.1994:22(12):2399 ... XuS Y http://biowww.net/methods/detail-132.html Nucleic Acids Research.1994:22(9):1764 ... KarikóK http://biowww.net/methods/detail-133.html Journal of Bacteriology.1993:175(17):5411 ... WoodgateR http://biowww.net/methods/detail-134.html Nucleic Acids Research.1993:21(22):5117 ... Ferguson-SmithM A http://biowww.net/methods/detail-135.html Journal of Bacteriology.1992:174(15):5171 ... GrussA