http://biowww.net/ Tue, 02 Dec 2008 09:29:45 +0100 FeedCreator 1.7.2 http://biowww.net/methods/detail-55.html A simple method for the isolation and subsequent detection of human immunodeficiency virus type 1 (HIV-1) RNA from feces is described. Viral RNA was isolated by the method developed by Boom et al. (R. Boom, C.J.A. Sol, M.M.M. Salimans, C.L. Jansen, P.M.E. We ... SolC J http://biowww.net/methods/detail-56.html Nucleic Acids Research.1993:21(20):4852 ... SiessW http://biowww.net/methods/detail-57.html Nucleic Acids Research.1993:21(8):2016 ... SchmelzerC http://biowww.net/methods/detail-58.html Nucleic Acids Research.1992:20(9):2381 ... JohnM E http://biowww.net/methods/detail-59.html Nucleic Acids Research.1991:19(21):6055 ... SeemannJ R http://biowww.net/methods/detail-60.html Nucleic Acids Research.1991:19(14):4011 ... OprandyJ J http://biowww.net/methods/detail-61.html We describe a simple method for the affinity purification of specific RNA-binding proteins. DNA sequences corresponding to the protein-binding site of the RNA are subcloned into an in vitro transcription vector between the T7 viral promoter and a poly(A) tra ... KühnL C http://biowww.net/methods/detail-62.html Nucleic Acids Research.1988:16(22):10933 ... WilkinsonM http://biowww.net/methods/detail-63.html Nucleic Acids Research.1988:16(22):10934 ... WilkinsonM http://biowww.net/methods/detail-64.html We report here a method of RNA preparation that may enrich for precursor RNA sequences and the results of an investigation of adeno-associated virus (AAV) RNA transcription that used this method. Whole cells were lysed with detergent and high salt and separa ... CarterB J http://biowww.net/methods/detail-65.html We present a rapid and efficient method for the detection of related transcripts with different expression levels. This approach combines the rapid amplification of cDNA ends (RACE) method with a cDNA subtractive technique. The strategy is based on successiv ... NeuvilleP http://biowww.net/methods/detail-66.html An efficient method for the isolation of human immunodeficiency virus type 1 (HIV-1) nucleic acids from dry cervical swabs was developed. HIV-1 Journal of Clinical Microbiology.1998:36(8):2349 ... OverbaughJulie http://biowww.net/methods/detail-67.html Nucleic Acids Research.1988:16(5 Pt B):2340 ... MeierR http://biowww.net/methods/detail-68.html Nucleic Acids Research.1988:16(22):10934 ... WilkinsonM http://biowww.net/methods/detail-69.html An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantl ... RemaleyAlan T. http://biowww.net/methods/detail-70.html A new one-step procedure for the isolation of bacterial RNA, involving lysis by proteinase K in the presence of sodium dodecyl sulfate, is described. Pulse-labeled RNA isolated by this procedure for Bacillus brevis, Bacillus subtilis, and Escherichia coli B ... SarkarN http://biowww.net/methods/detail-71.html The use of mercurated "strong stop" complementary DNA (complementary to the 5'-terminal 101 nucleotides of Rous sarcoma virus RNA) in the isolation of virus-specific RNA from infected chicken embryo fibroblasts is described. Strong stop Rous sarcoma virus co ... DarlixJ L http://biowww.net/methods/detail-72.html We describe an affinity chromatography method to isolate specific RNAs and RNA-protein complexes formed in vivo or in vitro. It exploits the highly selective binding of the coat protein of bacteriophage R17 to a short hairpin in its genomic RNA. RNA containi ... WickensM http://biowww.net/methods/detail-73.html Here we describe a reliable method for purifying large amounts of RNA of any sequence and length with comparable efficiency and resolution of gel electrophoresis and with capacity approaching that of column chromatography. The RNA mixture of interest is sepa ... LuY http://biowww.net/methods/detail-74.html Sephadex-binding RNA ligands (aptamers) were obtained through Nucleic Acids Research.2000:29(2):e4 ... EngelkeDavid R. http://biowww.net/methods/detail-75.html Nucleic Acids Research.1991:19(10):2786 ... SöllD http://biowww.net/methods/detail-76.html Several DNA/RNA sequencing strategies have been developed using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). In the reverse Sanger sequencing approach α-thiophosphate-containing NTPs are employed. Sequencing ladde ... HillenkampFranz http://biowww.net/methods/detail-77.html BMC Genomics.1994:91():5695 ... HiguchiR http://biowww.net/methods/detail-79.html Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for want of a poly(A) tail on these messages. We have used poly(A) polymerase from yeast to effect specific polyadenylation of Escherichia coli polysomal mRNA in the pre ... VijayaS http://biowww.net/methods/detail-80.html Improvements in the synthesis, deprotection and purification of oligoribonucleotides are described. These advances allow for reduced synthesis and deprotection times, while improving product yield. Coupling times are reduced by half using 5-ethylthio-1H-tetr ... UsmanN http://biowww.net/methods/detail-81.html The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2 (U* = [5'-2H]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60 degrees C. This procedure provided pure RNA dod ... OrbanJ