http://biowww.net/ Mon, 01 Dec 2008 22:39:17 +0100 FeedCreator 1.7.2 http://biowww.net/methods/detail-335.html Chromosomal rearrangements are frequently monitored by fluorescence in situ hybridization (FISH) using large, recombinant DNA probes consisting of contiguous genomic intervals that are often distant from disease loci. We developed smaller, targeted, single-cop ... KnollJoan H.M. http://biowww.net/methods/detail-336.html Nucleic Acids Research.2000:28(10):e46 ... JournotLaurent http://biowww.net/methods/detail-337.html Recently we developed a novel strategy for differentially painting all 24 human chromosomes. It is termed COBRA–FISH, short for combined binary ratio labeling–fluorescence in situ hybridization. COBRA–FISH is distinct from the pure comb ... MeltzerPaul http://biowww.net/methods/detail-338.html Determination of telomere length is traditionally performed by Southern blotting and densitometry, giving a mean telomere restriction fragment (TRF) value for the total cell population studied. Fluorescence in situ hybridization (FISH) of telomere repeats has ... RoosG http://biowww.net/methods/detail-339.html A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. Th ... SouthernE M http://biowww.net/methods/detail-340.html Fluorescence in situ hybridization to metaphase chromosomes or chromatin fibers in interphase nuclei is a powerful technique in mapping genes and DNA segments to specific chromosome region. We have been able to release the chromatin fibers from cells arrested ... TsuiL C http://biowww.net/methods/detail-341.html We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The fea ... RaapA K http://biowww.net/methods/detail-342.html Nucleic Acids Research.1990:18(9):2831 ... GagnéR http://biowww.net/methods/detail-343.html In fluorescent in situ hybridization (FISH), the efficiency of hybridization between the DNA probe and the rRNA has been related to the accessibility of the rRNA when ribosome content and cell permeability are not limiting. Published rRNA accessibility maps sh ... NogueraDaniel R.