Molecular Biology Methods Database RSS Feeds http://biowww.net/ Mon, 01 Dec 2008 22:25:09 +0100 FeedCreator 1.7.2 A novel method for constructing gene-targeting vectors http://biowww.net/methods/detail-344.html We developed a simple and rapid method for constructing knockout vectors using inverse-PCR (IPCR). The method consists of three steps: (i) digestion of a target bacterial artificial chromosome with several restriction enzymes (six-base cutters) followed bNu ... SasaiHitoshi A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations http://biowww.net/methods/detail-345.html Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.749203.Genome Research.2002:13(3):476 ... CopelandNeal G. A simplified method of generating transgenic Xenopus http://biowww.net/methods/detail-346.html Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has tNu ... MohunTim J. A method for the generation of YAC transgenic mice by pronuclear microinjection. http://biowww.net/methods/detail-347.html Yeast artificial chromosomes (YACs) represent the latest generation of vectors which have the great advantage of large insert size. The introduction of YACs into mammalian cells and organisms has become an important goal, since it offers the potential toNuc ... SchützG A rapid and economical method for screening transgenic mice. http://biowww.net/methods/detail-348.html Nucleic Acids Research.1989:17(9):3608 ... CoxK O A New Method for Rapidly Generating Gene-Targeting Vectors by Engineering BACs Through ... http://biowww.net/methods/detail-349.html Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.1356503. Article published online before print in August 2003.Genome Research.2003:13(9):2190 ... SnapperScott B.