http://biowww.net/ Tue, 02 Dec 2008 07:52:33 +0100 FeedCreator 1.7.2 http://biowww.net/methods/detail-300.html A practical fluorescence-labeling method for sequencing small RNAs by the traditional 'direct read out' on polyacrylamide gel electrophoresis was established. The 3' terminus of RNA was oxidized into dialdehyde by sodium periodate and then labeled with fluores ... LiuW Y http://biowww.net/methods/detail-301.html Nucleic Acids Research.1989:17(11):4414 ... PenaS D http://biowww.net/methods/detail-302.html Ribozyme reaction site mapping by Nucleic Acids Research.2003:13(4):88 ... RiefM. http://biowww.net/methods/detail-304.html Novel CE-phosphoramidite (7a-e) and CPG (8a, c, d, e) reagents have been prepared from a unique 2-aminobutyl-1,3-propanediol backbone. The reagents have been used to directly label oligonucleotides with fluorescein, acridine, and biotin via automated DNA synth ... MuthiniS http://biowww.net/methods/detail-308.html A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5′-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary h ... BergstromDonald E. http://biowww.net/methods/detail-309.html BMC Biotechnology.2002:30():e15 ... SpeedTP http://biowww.net/methods/detail-310.html The modification of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic ac ... AmackerMario http://biowww.net/methods/detail-311.html Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called ‘padlock oligonucleotide’. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous co ... EscudéChristophe http://biowww.net/methods/detail-312.html Two new photoreactive dATP analogs, Nucleic Acids Research.2000:28(21):4382 ... BartholomewBlaine http://biowww.net/methods/detail-313.html We report the design and evaluation of two non-nucleosidic photocleavable aminotag phosphor-amidites. These reagents introduce a photocleavable amino group on the 5'-terminal phosphate of synthetic oligonucleotides. The 5' photocleavable amino group enables in ... RothschildK J http://biowww.net/methods/detail-314.html A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends ... HusimiY http://biowww.net/methods/detail-315.html Automated DNA sequencing utilizing fluorescently labeled primers is a proven methodology for generating quality sequence data. However, for directed primer walking strategies this necessitates synthesis and labeling of a unique primer for each sequencing react ... SteffensD L http://biowww.net/methods/detail-316.html Site-specific labeling of covalently closed circular DNA was achieved by using triple helix-forming oligonucleotides 10, 11 and 27 nt in length. The sequences consisted exclusively of pyrimidines (C and T) with a reactive psoralen at the 5'-end and a biotin at ... LangowskiJ http://biowww.net/methods/detail-317.html Several procedures have been described for fluorescent labeling of DNA and RNA. They are based on the introduction of aldehyde groups by partial depurination of DNA or oxidation of the 3'-terminal ribonucleoside in RNA by sodium periodate. Fluorescent labels w ... MirzabekovA http://biowww.net/methods/detail-318.html A typing method for bacteria was developed and applied to several species, including Escherichia coli and Actinobacillus actinomycetemcomitans. Total genomic DNA was digested with a restriction endonuclease, and fragments were enabled with [alpha-32P]dATP by u ... PlasterkR H http://biowww.net/methods/detail-319.html Fluorescence in situ hybridization (FISH) has become and indispensable tool in a variety of areas of research and clinical diagnostics. Many applications demand an approach for simultaneous detection of multiple target sequences that is rapid and simple, yet s ... WaggonerA S http://biowww.net/methods/detail-320.html Two commonly used methods to end-label RNA-molecules are 5'-end labeling by polynucleotide kinase and 3'-end labeling with pCp and T4 RNA ligase. We show here that RNA 3'-ends can also be labeled with the chain-terminating analogue cordycepin 5'-triphosphate ( ... KellerW http://biowww.net/methods/detail-321.html We isolated aminoacyl-tRNA (60-70% yield) from human and rat tissues and measured, by GC/MS, its labeling in vivo by [15N]- and [13C]leucine. Tracer dilution artifacts seemed unlikely since, after infusion of L-[1-13C,15N]leucine into rats, (i) muscle leucyl-t ... RennieM J http://biowww.net/methods/detail-322.html The effect of tritiated thymidine incorporation on DNA replication was studied in Chinese hamster ovary cells. Rapidly eluting (small) DNA from cells labeled with 2 microCi of [3H]thymidine per ml (200 microCi/mmol) for 60 min matured to a large nonelutable si ... SchimkeR T http://biowww.net/methods/detail-323.html Nucleic Acids Research.1990:18(19):5909 ... WinnackerE L http://biowww.net/methods/detail-324.html We have developed a system for the enzymatic in vitro synthesis of non-radioactively labeled RNA which is derivatized with the hapten digoxigenin (DIG). The labeling reaction as well as the conditions for hybridization and detection of hybrids by an antibody-c ... KesslerC http://biowww.net/methods/detail-325.html A probe-free method was developed to detect DNA rearrangement in bacteria based on the electrophoretic separation of twice-digested restriction fragments of genomic DNA into a two-dimensional (2-D) pattern. The first restriction enzyme digestion was done in so ... Ts'oP O http://biowww.net/methods/detail-326.html A series of dATP and dCTP nucleotide analogs have been synthesized which are modified by attachment of aliphatic linkers containing a functional group to the amino-nitrogen at the hydrogen bonding positions of the bases, that is, at the 6-position of adenine a ... KlevanL http://biowww.net/methods/detail-327.html We describe a simple method for 3'-end labeling RNAs of known sequence. A short DNA template is designed to anneal to the 3'-end of the RNA, with a two nucleotide 5' overhang of 3'-TA-5', 3'-TG-5' or 3'-TC-5'. The Klenow fragment of DNA polymerase I can then c ... SzostakJ W