http://biowww.net/ Tue, 02 Dec 2008 13:12:59 +0100 FeedCreator 1.7.2 http://biowww.net/methods/detail-219.html We have developed a simple, PCR-based protocol, random primed/anchored-PCR (RPA-PCR), that allows the selective amplification and efficient cloning of segments that are adjacent to any known sequence. We demonstrate that RPA-PCR can be used to prepare a nested ... HughesS H http://biowww.net/methods/detail-220.html Nucleic Acids Research.1991:19(10):2785 ... TeradaM http://biowww.net/methods/detail-221.html Nucleic Acids Research.1991:19(14):4012 ... ReidK B http://biowww.net/methods/detail-222.html Site-directed, Ligase-Independent Mutagenesis (SLIM) is a novel PCR-mediated mutagenesis approach that can accommodate all three sequence modification types (insertion, deletion and substitution). The method utilizes an inverse PCR amplification of the templat ... TillettDaniel http://biowww.net/methods/detail-223.html We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of prime ... ReymondJean-Louis http://biowww.net/methods/detail-224.html Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. However, most current protocols require the target DNA to be cloned into a plasmid vector before mutagenesis can be performed, and none of them are effective for mu ... DongQihan http://biowww.net/methods/detail-226.html Nucleic Acids Research.2000:28(16):e78 ... MiyawakiAtsushi http://biowww.net/methods/detail-227.html A rapid method is described to efficiently perform site-directed mutagenesis based on overlap extension polymerase chain reaction (OE-PCR). Two template DNA molecules in different orientations relative to only one universal primer were amplified in parallel. B ... JaegerK E http://biowww.net/methods/detail-228.html We describe a highly efficient procedure for site-specific mutagenesis of double-stranded plasmids. The method relies on a single PCR primer which incorporates both the mutations at the selection site and the desired single base substitutions at the mutant sit ... ScalaG http://biowww.net/methods/detail-229.html We describe a rapid and efficient megaprimer PCR procedure for site-directed mutagenesis that does not require any intermediate purification of DNA between the two rounds of PCR. This protocol is based on the design of forward and reverse flanking primers with ... MadisonE L http://biowww.net/methods/detail-230.html Nucleic Acids Research.1996:24(7):1378 ... LowP S http://biowww.net/methods/detail-231.html Journal of Clinical Investigation.1996:97(9):1999 ... MarthJ D http://biowww.net/methods/detail-232.html Nucleic Acids Research.1995:23(9):1642 ... DevignesM D http://biowww.net/methods/detail-233.html Nucleic Acids Research.1994:22(3):541 ... StunnenbergH G http://biowww.net/methods/detail-234.html Nucleic Acids Research.1993:21(9):2277 ... LapeyreJ N http://biowww.net/methods/detail-235.html A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmids, bacterial strains or reagents is described. Only one synthetic oligonucleotide for each mutation is required, sub ... PestkaS http://biowww.net/methods/detail-236.html Nucleic Acids Research.1992:20(4):922 ... KaberdinV R http://biowww.net/methods/detail-237.html Nucleic Acids Research.1992:20(5):1147 ... ShawP E http://biowww.net/methods/detail-238.html Nucleic Acids Research.1992:20(19):5241 ... GaliliU http://biowww.net/methods/detail-239.html Nucleic Acids Research.1991:19(16):4558 ... de VosW M http://biowww.net/methods/detail-240.html Nucleic Acids Research.1990:18(16):4947 ... SpritzR A http://biowww.net/methods/detail-241.html We have generated a site-specific 17 bp insertion within a 38 kb chick globin gene cluster by employing the recombination abilities of Saccharomyces cerevisiae. This gene cluster contains four beta-type globin genes which share a high degree of sequence homolo ... EmersonB M http://biowww.net/methods/detail-242.html Nucleic Acids Research.1990:18(24):7457 ... PerlakF J http://biowww.net/methods/detail-243.html Nucleic Acids Research.1990:18(10):3077 ... RiegerA http://biowww.net/methods/detail-244.html A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer whic ... ThompsonD V http://biowww.net/methods/detail-245.html Nucleic Acids Research.1989:17(13):5408 ... KunkelT A http://biowww.net/methods/detail-246.html To generate a random mutant library that is free from mutation at a particular amino acid residue, we replace the codon of interest with a detachable, short DNA sequence containing a BsaXI recognition site. After PCR mutagenesis, this sequence is removed and i ... ArnoldFrances H. http://biowww.net/methods/detail-247.html We describe codon cassette mutagenesis, a simple method of mutagenesis that uses universal mutagenic cassettes to deposit single codons at specific sites in double-stranded DNA. A target molecule is first constructed that contains a blunt, double-strand break ... DiMaioD http://biowww.net/methods/detail-248.html We describe a new procedure to identify RNA or DNA binding sites in proteins, based on a combination of UV cross-linking and single-hit chemical peptide cleavage. Site-directed mutagenesis is used to create a series of mutants with single Asn-Gly sequences in ... KühnL C http://biowww.net/methods/detail-249.html Nucleic Acids Research.1993:21(3):777 ... KuipersO P http://biowww.net/methods/detail-250.html Nucleic Acids Research.1990:18(17):5323 ... MaloM S http://biowww.net/methods/detail-251.html Nucleic Acids Research.1989:17(14):5862 ... FritzH J http://biowww.net/methods/detail-252.html A simple and efficient procedure for the generation of random GC to AT transition mutations in a specific DNA segment is described. A restriction fragment is inserted in each orientation into an M13 vector, single-stranded virion DNA from each recombinant phag ... KnowlesJ R http://biowww.net/methods/detail-253.html A protocol has been developed that is capable of saturating regions hundreds of basepairs in length with linker scanning mutations. The efficacy of this method stems from the design of the linker scanning mutagenesis (LSM) cassette which is composed of a selec ... LinnT http://biowww.net/methods/detail-254.html 'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made wi ... ShapiroL J