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Minimal Medium M9

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1. Add 1 g NH4Cl and 3 g glucose in 100 ml 10X M9 Salts salution. 2. Add 1 ml 1 M MgSO4 and 10 ml Gibco MEM vitamin solution. 3. Make up volume to 1 liter with distilled water. 4. Check that the pH is 7.0-7.4. 5. Filter sterilize through a 0.22 micron filter. 6. Add 1 ml sterile 100 mM CaCl2 solution. Store at room temperature.
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