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inconsistent PCR products

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 I am working with 20x diluted soil DNA and sometimes I would get products and sometimes I wouldnt.  For several samples, I would get a product one time and not get it another.  This puzzles me because I always make sure all the reagents are thawed and centrifuged before i use it and I always mix the master mix.  The template DNA is always thawed before i add it to the PCR mixtures, but my results are still inconsistent and I would like to know how to solve this problem!
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