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PCR Product is not running into an agarose gel

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hi!

i am facing a problem with a pcr product of dna from a Whole Genome Amplification. i have 6 samples at all, and 5 are working well, that means, they are running into the agarose gel, but one ist sticking in the well...and i do not really know why?

i tried purification with exosap and microcon, but with the same result?

can anyone help me?

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my setting:
- sample: Whole Genome Amplification dna
- pcr with GoTaq® Green Master Mix
- ordinary agarose gel (1.5% w/v)
total 539 hits     Asked by: Anonymouse I want to answer  


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