Online life science pharma marketplace and platform for products and services.


Current location: Home » Ask » Immunology and cytometry » Content

immunocytochemistry protocol for biotinylated primary antibody

Pending answer Credit reward to answer the question: 0 -
More information:
This is going to be the first immunocytochemistry I perform, and I'd like to ask for advice first. I'll determine the relative levels of alpha2beta1 integrin in different human cell lines.
The lab manager ordered a primary antibody that has biotin attached already. All the protocols I compiled call for a secondary antibody -evidently I'll have to change them.
What difference does it make if I use a primary antibody with a biotin tag in regards to signal-to-noise ratio, sensitivity, etc? What should I use as controls, and what kind of blocking steps would be necessary?

Any advice is greatly appreciated (in immunocytochemistry in general as well...).
Thank you.

total 949 hits     Asked by: Anonymouse I want to answer  

[ AskSearch ]  [ ]  [ Tell a friend ]  [ Print ]  [ Close window ]  [ Return to top ]

Search ask
Related questions
Questions pending your answer